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HPLC Analysis of Cytochrome c Species Variants on Discovery® BIO PolyMA-SCX (Ion Exchange)

HPLC Analysis of Cytochrome c Species Variants on Discovery® BIO PolyMA-SCX (Ion Exchange) application for HPLC

材料

標準

製品番号
詳細
価格

C4011

Cytochrome c from pigeon breast muscle

≥95% based on Mol. Wt. 12,173 basis

C2037

シトクロムC ウシ心臓由来

≥95% based on Mol. Wt. 12,327 basis

C3131

シトクロムC ウシ心臓由来

≥95% based on Mol. Wt. 12,327 basis, powder, suitable for mammalian cell culture

C7150

シトクロムC from equine heart

BioReagent, suitable for GFC marker

C7752

シトクロムC from equine heart

≥95% based on Mol. Wt. 12,384 basis

C2506

シトクロムC from equine heart

≥95% (SDS-PAGE)

C2436

シトクロムC from Saccharomyces cerevisiae

≥85% based on Mol. Wt. 12,588 basis

C3483

チトクロームc ヒト心臓由来

≥95% (SDS-PAGE), lyophilized powder

30398

チトクロームC ウシ心臓由来

≥95% (GE)

C2867

シトクロムC from equine heart

BioUltra, ≥99% (SDS-PAGE), powder, suitable for mammalian cell culture

CONDITIONS

column

Discovery BIO PolyMA-SCX, 5 cm x 4.6 mm, 5 μm particles (59601-U)

mobile phase

[A] 20 mM Bis-Tris HCl, pH 7.0; [B] 20 mM Bis-Tris HCl, 0.5M NaCl, pH 7.0; 24 to 69% B in 20 min. (linear)

flow rate

0.5 mL/min

column temp.

25 °C

detector

UV, 280 nm

injection

10 μg each variant

詳細

アナリシスノート

This Figure shows the separation of three cytochrome c variants on Discovery BIO PolyMA-SCX. Note the difference in elution order compared to the reversed-phase separation on Discovery BIO Wide Pore C18 shown in product G001581.

法的情報

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

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Related Product Categories

HPLC ColumnsGC Columns & AccessoriesTLC Plates & AdsorbentsSolid Phase Microextraction (SPME)Solid Phase Extraction & QuEChERS

Related Applications

Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS