HPLCによる塩基性ペプチドの分析、Ascentis Express Peptide ES-C18

column

BIOshell A160 Peptide C18, 10 cm x 3 mm I.D., 2.7 μm particles (66908-U)

mobile phase

[A] 0.1% (v/v) formic acid, pH 4.0 (titrated with ammonium hydroxide); [B] 50:50, (0.175% formic acid in water, pH 4.0) : acetonitrile

gradient

20 to 50% B in 15 min

flow rate

0.6 mL/min

pressure

3002 psi (207 bar)

column temp.

35 °C

detector

UV, 215 nm

injection

2.0 μL

sample

0.5 g/L ea. peptide