HPLCによる尿中のアンフェタミンの分析、 Discovery^® HS F5、SPE処理、Discovery^® DSC-MCAX および Standard C18使用

sample preparation

SPE (Solid Phase Extraction)

sample/matrix

human urine spiked amphetamine and methylamphetamine at with 2 mg/mL

SPE tube/cartridge

Discovery DSC-MCAX, 100 mg/3mL (52783-U)

condition

DSC-MCAX SPE tube: 1 mL methanol; 1 mL 50 mM ammonium acetate, pH 6.0; C-18 SPE tube: 1 mL methanol; 1 mL DI water

sample addition

1 mL

washing

DSC-MCAX SPE tube: 1 mL 50 mM ammonium acetate, pH 6.0; 1 mL 1M acetic acid; 1mL methanol; C-18 SPE tube: 1 mL DI water; 1 mL 20% methanol

elution

DSC-MCAX SPE tube: 1 mL 5% ammonium hydroxide in methanol; C-18 SPE tube: 1 mL methanol

eluate post-treatment

evaporate to dryness with nitrogen at room temperature, reconstitute in mobile phase

column

Discovery HS F5, 15 cm x 4.6 mm I.D., 5 μm particles (567516-U)

mobile phase

[A] 10 mM ammonium acetate, pH 4.5: [B] methanol; (35:65, A:B)

flow rate

2 mL/min

column temp.

40 °C

detector

UV, 210 nm

injection

10 μL