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  • The antipsychotic amisulpride: ultrastructural evidence of its secretory activity in salivary glands.

The antipsychotic amisulpride: ultrastructural evidence of its secretory activity in salivary glands.

Oral diseases (2013-11-20)
F Loy, M Isola, R Isola, M A Lilliu, P Solinas, G Conti, T Godoy, A Riva, J Ekström
要旨

Amisulpride is reported to inhibit clozapine-induced sialorrhea. Preclinically, clozapine evokes muscarinic-M1-type-mediated secretion that, however, amisulpride does not reduce. Instead, amisulpride, without causing any overt secretion per se, enhances both nerve- and autonomimetic-evoked salivation by unknown mechanism(s). Hypothesizing that amisulpride prepares the gland for secretion, we looked for ultrastructural events indicating secretory activity in intercellular canaliculi of serous/seromucous cells, that is, density increase in protrusions (reflecting anchored granules) and in microbuds (reflecting recycling membranes and/or vesicle secretion) and decrease in microvilli (reflecting the cytoskeletal re-arrangement related to exocytosis). Rat parotid and submandibular glands were exposed to amisulpride in vivo or in vitro. Glands were processed for transmission electron and scanning electron microscopy and then morphometrically assessed. Cells were packed with secretory granules. The density of protrusions increased in both glands, whereas significant and parallel changes in microvilli and microbuds occurred only in parotid glands, and in vitro. Amisulpride induced ultrastructural signs of secretory activity but to varying extent; in submandibular glands, in contrast to parotid glands, changes were not brought beyond the granular anchoring stage. Amisulpride may provide an overall readiness for secretion that will result in augmented responses to agonists, a phenomenon of potential interest in dry-mouth treatment.

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製品内容

Sigma-Aldrich
(±)-プロプラノロール 塩酸塩, ≥99% (TLC), powder
Sigma-Aldrich
アトロピン 硫酸塩 一水和物, ≥97% (TLC), crystalline
Sigma-Aldrich
メタンスルホン酸, ≥99.0%
USP
アトロピン 硫酸塩 一水和物, United States Pharmacopeia (USP) Reference Standard
USP
(±)-プロプラノロール 塩酸塩, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
Amisulpride, ≥98% (HPLC)
Sigma-Aldrich
メタンスルホン酸 溶液, 70 wt. % in H2O
Supelco
メタンスルホン酸, suitable for HPLC, LiChropur, ≥99.5% (T)
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アトロピン 硫酸塩 一水和物, Pharmaceutical Secondary Standard; Certified Reference Material
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(±)-プロプラノロール 塩酸塩, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
メタンスルホン酸濃縮物, 0.1 M CH3SO3H in water (0.1N), eluent concentrate for IC
(±)-プロプラノロール 塩酸塩, European Pharmacopoeia (EP) Reference Standard
Supelco
(±)-プロプラノロール 塩酸塩, analytical standard
アトロピン 硫酸塩 一水和物, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
メタンスルホン酸 溶液, 4 M (with 0.2% (w/v) tryptamine)
Amisulpride, European Pharmacopoeia (EP) Reference Standard
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