- A DNA-based molecular probe for optically reporting cellular traction forces.
A DNA-based molecular probe for optically reporting cellular traction forces.
Nature methods (2014-10-13)
Brandon L Blakely, Christoph E Dumelin, Britta Trappmann, Lynn M McGregor, Colin K Choi, Peter C Anthony, Van K Duesterberg, Brendon M Baker, Steven M Block, David R Liu, Christopher S Chen
PMID25306545
要旨
We developed molecular tension probes (TPs) that report traction forces of adherent cells with high spatial resolution, can in principle be linked to virtually any surface, and obviate monitoring deformations of elastic substrates. TPs consist of DNA hairpins conjugated to fluorophore-quencher pairs that unfold and fluoresce when subjected to specific forces. We applied TPs to reveal that cellular traction forces are heterogeneous within focal adhesions and localized at their distal edges.
材料
製品番号
ブランド
製品内容
Sigma-Aldrich
ジメチルスルホキシド, Hybri-Max™, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%
Sigma-Aldrich
ジメチルスルホキシド, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma
Sigma-Aldrich
L-グルタミン, meets USP testing specifications, suitable for cell culture, 99.0-101.0%, from non-animal source