コンテンツへスキップ
Merck
  • The antibiofilm activity of lingonberry flavonoids against oral pathogens is a case connected to residual complexity.

The antibiofilm activity of lingonberry flavonoids against oral pathogens is a case connected to residual complexity.

Fitoterapia (2014-06-01)
Kaisu R Riihinen, Zhen M Ou, Tanja Gödecke, David C Lankin, Guido F Pauli, Christine D Wu
要旨

The antimicrobial activity of lingonberry (Vaccinium vitis-idaea L.) was evaluated against two oral pathogens, Streptococcus mutans and Fusobacterium nucleatum. Long-bed gel permeation chromatography (GPC; Sephadex LH-20) yielded purified flavonoids, with the most efficient minimum inhibitory concentrations (MICs) against planktonic cells in the anthocyanin and procyanidin primary fractions against F. nucleatum (63-125 μg/ml) and in the procyanidin rich fraction against S. mutans (16-31 μg/ml). The purified flavonol glycosides and procyanidins inhibited biofilm formation of S. mutans (MICs 16-31 μg/ml), while the corresponding reference compounds showed no activity. Secondary GPC purification yielded flavonol glycosides devoid of antibiofilm activity in the 50% MeOH fraction, while elution with 70% acetone recovered a brownish material with activity against S. mutans biofilm (MIC 8 μg/ml). Even after HPLC-PDA, NMR, and MALDI-TOF analyses, the structural identity of this material remained unknown, while its color and analytical characteristics appear to be consistent with flavonoid oxidation products.

材料
製品番号
ブランド
製品内容

Sigma-Aldrich
ケルセチン, ≥95% (HPLC), solid
Sigma-Aldrich
カフェイン酸, ≥98.0% (HPLC)
Sigma-Aldrich
p-クマル酸, ≥98.0% (HPLC)
Sigma-Aldrich
クロロゲン酸, ≥95% (titration)
Sigma-Aldrich
trans-フェルラ酸, 99%
Sigma-Aldrich
バニリン酸, 97%
USP
ケルセチン, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
trans-フェルラ酸, ≥99%
Sigma-Aldrich
ケルセチン 3-D-ガラクトシド, ≥97.0% (HPLC)
Sigma-Aldrich
バニリン酸, ≥97%, FG
Supelco
ケルセチン, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
バニリン酸, purum, ≥97.0% (HPLC)
Supelco
trans-フェルラ酸, matrix substance for MALDI-MS, ≥99.0% (HPLC)
クロロゲン酸, primary reference standard
Supelco
p-クマル酸, analytical standard
Sigma-Aldrich
Idaein chloride, ≥90% (HPLC)
trans-フェルラ酸, European Pharmacopoeia (EP) Reference Standard
ヒペロシド, primary reference standard
Supelco
カフェイン酸, matrix substance for MALDI-MS, ≥99.0% (HPLC)
Supelco
プロシアニジン B2, analytical standard
Supelco
カフェイン酸, matrix substance for MALDI-MS, ≥99.0% (HPLC), powder, light beige
クロロゲン酸, European Pharmacopoeia (EP) Reference Standard
Supelco
バニリン酸, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
ケルセチン 3-ラムノシド, primary reference standard
Supelco
trans-フェルラ酸, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland