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Merck

Hepatic stellate cells contribute to progenitor cells and liver regeneration.

The Journal of clinical investigation (2014-11-18)
Claus Kordes, Iris Sawitza, Silke Götze, Diran Herebian, Dieter Häussinger
要旨

Retinoid-storing hepatic stellate cells (HSCs) have recently been described as a liver-resident mesenchymal stem cell (MSC) population; however, it is not clear whether these cells contribute to liver regeneration or serve as a progenitor cell population with hepatobiliary characteristics. Here, we purified HSCs with retinoid-dependent fluorescence-activated cell sorting from eGFP-expressing rats and transplanted these GFP(+) HSCs into wild-type (WT) rats that had undergone partial hepatectomy in the presence of 2-acetylaminofluorene (2AAF) or retrorsine, both of which are injury models that favor stem cell-based liver repair. Transplanted HSCs contributed to liver regeneration in host animals by forming mesenchymal tissue, progenitor cells, hepatocytes, and cholangiocytes and elevated direct bilirubin levels in blood sera of GUNN rats, indicating recovery from the hepatic bilirubin-handling defect in these animals. Transplanted HSCs engrafted within the bone marrow (BM) of host animals, and HSC-derived cells were isolated from BM and successfully retransplanted into new hosts with injured liver. Cultured HSCs transiently adopted an expression profile similar to that of progenitor cells during differentiation into bile acid-synthesizing and -transporting hepatocytes, suggesting that stellate cells represent a source of liver progenitor cells. This concept connects seemingly contradictory studies that favor either progenitor cells or MSCs as important players in stem cell-based liver regeneration.

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Sigma-Aldrich
メタノール, suitable for HPLC, ≥99.9%
Sigma-Aldrich
メタノール, ACS reagent, ≥99.8%
Sigma-Aldrich
メタノール, suitable for HPLC, gradient grade, ≥99.9%
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メタノール, HPLC Plus, ≥99.9%
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DAPI, for nucleic acid staining
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ホルムアルデヒド 溶液, for molecular biology, 36.5-38% in H2O
Sigma-Aldrich
メタノール, anhydrous, 99.8%
Sigma-Aldrich
メタノール, suitable for HPLC, gradient grade, suitable as ACS-grade LC reagent, ≥99.9%
Sigma-Aldrich
メタノール, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8% (GC)
SAFC
ホルムアルデヒド 溶液, contains 10-15% methanol as stabilizer, 37 wt. % in H2O
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メタノール, Laboratory Reagent, ≥99.6%
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メタノール, BioReagent, ≥99.93%
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亜セレン酸ナトリウム, BioReagent, suitable for cell culture, ≥98%
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メタノール, Absolute - Acetone free
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ホルムアルデヒド 溶液, ACS reagent, 37 wt. % in H2O, contains 10-15% Methanol as stabilizer (to prevent polymerization)
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メタノール, ACS spectrophotometric grade, ≥99.9%
USP
メチルアルコール, United States Pharmacopeia (USP) Reference Standard
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亜セレン酸ナトリウム, 99%
Sigma-Aldrich
ホルムアルデヒド 溶液, for molecular biology, BioReagent, ≥36.0% in H2O (T)
Roche
ペプシン, lyophilized (salt-free), ~2500 units/mg protein (At 37 °C with hemoglobin as the substrate. One unit is the enzyme activity which liberates the amount of Tyr producing an increase in the absorbance of 0.001/minute at 280 nm.)