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Merck

Cellular and Molecular Probing of Intact Human Organs.

Cell (2020-02-16)
Shan Zhao, Mihail Ivilinov Todorov, Ruiyao Cai, Rami Ai -Maskari, Hanno Steinke, Elisabeth Kemter, Hongcheng Mai, Zhouyi Rong, Martin Warmer, Karen Stanic, Oliver Schoppe, Johannes Christian Paetzold, Benno Gesierich, Milagros N Wong, Tobias B Huber, Marco Duering, Oliver Thomas Bruns, Bjoern Menze, Jan Lipfert, Victor G Puelles, Eckhard Wolf, Ingo Bechmann, Ali Ertürk
要旨

Optical tissue transparency permits scalable cellular and molecular investigation of complex tissues in 3D. Adult human organs are particularly challenging to render transparent because of the accumulation of dense and sturdy molecules in decades-aged tissues. To overcome these challenges, we developed SHANEL, a method based on a new tissue permeabilization approach to clear and label stiff human organs. We used SHANEL to render the intact adult human brain and kidney transparent and perform 3D histology with antibodies and dyes in centimeters-depth. Thereby, we revealed structural details of the intact human eye, human thyroid, human kidney, and transgenic pig pancreas at the cellular resolution. Furthermore, we developed a deep learning pipeline to analyze millions of cells in cleared human brain tissues within hours with standard lab computers. Overall, SHANEL is a robust and unbiased technology to chart the cellular and molecular architecture of large intact mammalian organs.

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製品番号
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製品内容

Sigma-Aldrich
抗ラミニン ウサギ宿主抗体, 0.5 mg/mL, affinity isolated antibody, buffered aqueous solution
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Histodenz, nonionic density gradient medium
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N-メチルジエタノールアミン, ≥99%
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クレシルバイオレット酢酸, certified by the Biological Stain Commission
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アジ化ナトリウム, purum p.a., ≥99.0% (T)
Sigma-Aldrich
過ヨウ素酸シッフ(PAS)染色システム
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trans-シンナムアルデヒド, 97%
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メチル-β-シクロデキストリン, average Mn 1310
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Triton X-100, laboratory grade
BRAND® 96-well microplate, U-bottom, round bottom, non-sterile
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メチレンブルー, certified by the Biological Stain Commission
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酢酸ナトリウム, anhydrous, for molecular biology, ≥99%
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安息香酸ベンジル, ≥99%, FCC, FG
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trans-1-アセチル-4-ヒドロキシ-L-プロリン, ≥98%
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Monoclonal Anti-MBP antibody produced in mouse, Prestige Antibodies® Powered by Atlas Antibodies, clone CL2829, purified immunoglobulin, buffered aqueous glycerol solution
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ジベンジルエーテル, purum, ≥98.0% (GC)
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サリチル酸メチル, tested according to Ph. Eur.