G005675

HPLC Analysis of Benzodiazepines on Ascentis^® Express Phenyl-Hexyl with Acetonitrile Mobile Phase

application for HPLC

特性

• テクニック: HPLC: suitable
• 試験パラメータ: column: Ascentis Express Phenyl-Hexyl, 5 cm x 4.6 mm I.D., 2.7 μm particles (53348-U); mobile phase: [A] water; [B] acetonitrile; (62:38, A:B); flow rate: 1.4 mL/min; pressure: 2600 psi (179 bar); column temp.: 35 °C; detector: UV, 250 nm; injection: 2 μL; sample: 0.1 g/mL of each in 20% methanol
• 適合性: application for HPLC
• アプリケーション: clinical; pharmaceutical (small molecule)

詳細

アナリシスノート

The Phenyl-Hexyl phase of the Ascentis Express product line displays the best selectivity of this sample; selectivity is markedly affected by choice of organic modifier. The application also demonstrates feasibility of fast LC with Ascentis Express by virtue of low backpressure and high efficiency.

法的情報

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

アナライト

Oxazepam
Alprazolam
Clonazepam
Desmethyldiazepam
Diazepam