ZRB1602

Anti-Caveolin-1 Antibody, clone 1D10 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 別名: CAV-1
• UNSPSCコード: 12352203

特性

• 由来生物: rabbit
• 品質水準: 200
• リコンビナント: expressed in HEK 293 cells
• 結合体: unconjugated
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 1D10, recombinant monoclonal
• 詳細: recombinant, expressed in HEK 293 cells
• 製品種目: ZooMAb^® learn more
• 形状: lyophilized
• 分子量: calculated mol wt 20.47 kDa; observed mol wt ~21 kDa
• 精製方法: using Protein A
• 化学種の反応性: mouse, human, monkey, rat
• 包装: antibody small pack of 25 μL
• 環境により配慮した代替製品の特徴: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 強化検証: recombinant expression; Learn more about Antibody Enhanced Validation
• テクニック: affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• アイソタイプ: IgG
• エピトープ配列: N-terminal
• タンパク質IDアクセッション番号: NP_001744
• UniProtアクセッション番号: Q03135
• 環境により配慮した代替製品カテゴリ: , Aligned
• 輸送温度: ambient
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

特異性

Clone 1D10 is a Rabbit recombinant monoclonal antibody that specifically detects Caveolin-1. It targets an epitope within 19 amino acids from the N-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 19 amino acids from the N-terminal region of human Caveolin-1.

アプリケーション

Quality Control Testing

Evaluated by Western Blotting in C6 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Caveolin-1 in C6 cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Caveolin-1 in lysates from Cos-7 and HeLa cells and from Mouse Lung tissue.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Caveolin-1 in A431, HeLa, and NIH3T3 cells.

Affinity Binding Assay: A representative lot of this antibody bound Caveolin-1 peptide with a KD of 7.3 x 10-8 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Caveolin-1 in human liver tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

ターゲットの説明

Caveolin-1 (UniProt: Q03135) is encoded by the CAV1 (also known as CAV) gene (Gene ID: 857) in human. Three caveolin genes are expressed in mammals and are designated as caveolin-1, -2, and -3. Most mammalian tissues express at least one of these isoforms. Caveolin-1 is expressed in skeletal muscle, liver, stomach, lung, brain, kidney and heart. Caveolin-1 and -2 are usually co-expressed and assemble into hetero-oligomers in the endoplasmic reticulum and Golgi apparatus. Two isoforms of caveolin-1 have been reported that are produced by alternative initiation. It has two cytoplasmic domains (aa 2-104 and 126-178) and an intramembrane domain (aa 105-125). The primary structure of caveolin-1 contains two serine residues, both residing in a phosphorylation consensus sequence and flanked by a regulatory scaffolding domain. Serine 37 site represents a PKC consensus site, while Serine 88 is believed to be phosphorylated by CK II alpha. Caveolin-1 acts as a scaffolding protein within caveolar membranes and is shown to interact directly with G-protein alpha subunits to functionally regulate their activity. It is also involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. It can bind to DPP4 and induce T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Mutations in CAV1 gene are known to cause Congenital generalized lipodystrophy 3 that is characterized by a near complete absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, and early onset of diabetes. Some mutations have also been linked to primary pulmonary hypertension with plexiform lesions of proliferating endothelial cells in pulmonary arterioles leading to elevated pulmonary arterial pression, right ventricular failure, and death. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Liu, P., et al. (2002). J. Biol. Chem. 277 (44); 41295-41298; Garver, WS., et al. (1999). Biochem. Biophys. Acta 143 (1453); 193-206).

物理的形状

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

再構成

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

保管および安定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法的情報

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 13 - Non Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable