ZMS1158

Anti-Collagen Type VII Antibody, clone NP32 ZooMAb^® Mouse Monoclonal

recombinant, expressed in HEK 293 cells

• 別名: LC collagen, Long-chain collagen
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• リコンビナント: expressed in HEK 293 cells
• 結合体: unconjugated
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: NP32, recombinant monoclonal
• 製品種目: ZooMAb^® learn more
• 形状: lyophilized
• 分子量: calculated mol wt 295.22 kDa; observed mol wt ~300 kDa
• 精製方法: using protein G
• 化学種の反応性: human
• 化学種の反応性（ホモロジーによる予測）: bovine
• 包装: antibody small pack of 25 μL
• 環境により配慮した代替製品の特徴: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 強化検証: recombinant expression; Learn more about Antibody Enhanced Validation
• テクニック: immunocytochemistry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• アイソタイプ: IgG1κ
• エピトープ配列: N-terminal half
• タンパク質IDアクセッション番号: NM_000094.3
• UniProtアクセッション番号: Q02388
• 環境により配慮した代替製品カテゴリ: Aligned
• 輸送温度: ambient
• 保管温度: 2-8°C
• 遺伝子情報: human ... COL7A1(1294)

詳細

詳細

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

特異性

Clone NP32 is a ZooMAb^® mouse recombinant monoclonal antibody that specifically detects Collagen alpha-1(VII) chain. It targets an epitope within the NC-1 domain from the N-terminal half.

免疫原

Type VII collagen from Human chorioamniotic membranes.

アプリケーション

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Collagen Type VII in HeLa cell lysate.

Tested Applications

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Collagen Type VII in HeLa cells.

Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Collagen Type VII in human frozen skin tissue sections.

Immunohistochemistry Applications: A representative lot detected Collagen Type VII in human skin tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

ターゲットの説明

Collagen alpha-1(VII) chain (UniProt: Q02388; also known as Long-chain collagen, LC collagen) is encoded by the COL7A1 gene (Gene ID: 1294) in human. Collagen is the most abundant protein in the body that is predominantly synthesized by fibroblasts. However, epithelial cells may also synthesize small amounts of collagen. The collagen super family includes over 20 different types of collagens with at least 38 distinct polypeptide chains. Collagen type VII is a homotrimeric protein that is primarily synthesized by keratinocytes and skin fibroblasts. It is composed of three identical alpha chains and its distribution is restricted to stratified squamous epithelial basement membrane. It contributes to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins. Type VII collagen contains a non-collagenous domain 1 (NC1), a central collagenous domain, and a non-collagenous domain 2 (NC2) that catalyze the anchoring fibril assembly. Mutations in COL7A1 gene have been linked to epidermolysis bullosa dystrophica, a blistering skin disease characterized by tissue separation that occurs below the dermal-epidermal basement membrane at the level of the anchoring fibrils. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.  (Ref.: Supp, DM., et al. (2019). Cell Transp. 28(9-10); 1242-1256; Heagerty, AHM., et al. (1986). J. Invest. Dermatol. 86(5); 603-605). 

物理的形状

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

再構成

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

保管および安定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable