ZMS1064

Anti-Dynein Antibody, clone 74.1 ZooMAb^® Mouse Monoclonal

recombinant, expressed in HEK 293 cells

• UNSPSCコード: 12352203

特性

• 由来生物: mouse
• 品質水準: 200
• リコンビナント: expressed in HEK 293 cells
• 結合体: unconjugated
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 74.1, recombinant monoclonal; monoclonal
• 製品種目: ZooMAb^® learn more
• 形状: lyophilized
• 分子量: calculated mol wt ~68.56 kDa; observed mol wt ~78 kDa
• 化学種の反応性: rat, human, mouse
• 化学種の反応性（ホモロジーによる予測）: Xenopus, bovine, sheep, fish, Drosophila
• 包装: antibody small pack of 25 μL
• 環境により配慮した代替製品の特徴: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 強化検証: recombinant expression; Learn more about Antibody Enhanced Validation
• テクニック: flow cytometry: suitable; immunocytochemistry: suitable; western blot: suitable
• アイソタイプ: IgG2b
• UniProtアクセッション番号: Q29RQ3
• 環境により配慮した代替製品カテゴリ: Aligned,
• 輸送温度: ambient
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

特異性

Clone 74.1 is a ZooMAb^® mouse recombinant monoclonal antibody that specifically detects cytoplasmic dynein.

免疫原

Purified native bovine brain cytoplasmic dynein.

アプリケーション

Quality Control Testing

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Dynein in HEK293 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Dynein in A431 and PC12 cell lysate.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Dynein in HeLa, HepG2, and NIH 3T3 cells.

Flow Cytometry Analysis: 0.1 μg from a representative lot detected Dynein in one million HeLa cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Dynein, clone 74.1 ZooMAb^®, Cat. No. ZMS1064, is a recombinant Mouse monoclonal antibody that specifically targets Dynein and is tested for use in Flow Cytometry, Immunocytochemistry, and Western Blotting.

ターゲットの説明

Cytoplasmic dynein 1 intermediate chain 1 (UniProt: Q29RQ3; also known as Cytoplasmic dynein intermediate chain 1, Dynein intermediate chain 1, cytosolic, DH IC-1) is encoded by the DYNC1I1 gene (Gene ID: 613724) in bovine. Cytoplasmic dynein complexes are microtubules-associated retrograde transport motors. Cytoplasmic dynein 1 It is the more abundant motor in cells, while cytoplasmic dynein 2 takes part in intraflagellar transport. It is a multi-subunit complex of 1.5 MDa composed of a homodimer of heavy chains, two intermediate chains, light-intermediate chains, and light chains. The heavy chain homodimer acts as the dynein complex core and binds microtubules to enable ATP-dependent cytoplasmic dynein movement. The other dynein subunits associate as homodimers and play regulatory roles in maintaining the complex stability, modulating its activity, and mediating its interaction with accessory and cargo proteins. Cytoplasmic dynein 1 intermediate chain 1 acts as one of the several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are involved in linking dynein to cargos and to adapter proteins that regulate dynein function. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

物理的形状

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

再構成

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

保管および安定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法的情報

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable