T8535

モノクローナル抗β-チューブリンI + II抗体 マウス宿主抗体

clone JDR.3B8, ascites fluid

• 別名: Anti-CDCBM5, Anti-TUBB, Anti-TUBB2
• MDL番号: MFCD00145892
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: ascites fluid
• 抗体製品タイプ: primary antibodies
• クローン: JDR.3B8, monoclonal
• 含みます: 15 mM sodium azide
• 化学種の反応性: bovine, pig, chicken, human, rat, mouse
• 包装: antibody small pack of 25 μL
• テクニック: indirect ELISA: suitable; indirect immunofluorescence: suitable; microarray: suitable; western blot: 1:1,000 using a chicken fibroblast extract or a chicken brain microtubule proteins preparation
• アイソタイプ: IgG2b
• UniProtアクセッション番号: Q13885; Q9H4B7
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... TUBB1(81027) , TUBB2A(7280) , TUBB2C(10383) ; mouse ... Tubb1(545486) , Tubb2a(22151) , Tubb2c(227613) ; rat ... Tubb2b(291081) , Tubb2c(296554)

詳細

詳細

β-tubulin belongs to the subfamily of tubulins, which are the major building blocks of microtubules. β-tubulin has a molecular weight of 55kDa. β-tubulin structure is characterized with a core of two β-sheets enclosed by α-helices. In addition, it also contains an N-terminal domain with the guanine nucleotide-binding region, an intermediate domain with taxol-binding site and a C-terminal domain that contains the binding surface for molecular motor proteins. β-tubulin I and II is encoded by the gene mapped to human chromosome 20 and 6p25.2 respectively.

Monoclonal Anti-β-Tubulin Isotype I + II (mouse IgG2b isotype) is derived from the JDR.3B8 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice.

特異性

本抗体は、β-チュ-ブリンアイソタイプ I, II に存在するエピト-プを特異的に認識します。他のチュ-ブリンアイソタイプとの交差反応性は認められません。

免疫原

ヒトβ-チュ-ブリンアイソタイプII のC末端配列に相当する合成ペプチドのBSA結合体。

アプリケーション

Monoclonal Anti-β-Tubulin I+II antibody produced in mouse has been used in following studies:

• immunoblot
• immunofluorescence technique
• immunohistochemistry.

Monoclonal Anti-β-Tubulin I+II antibody produced in mouse has been used in:

• enzyme linked immunosorbent assay (ELISA)
• immunohistochemistry
• western blotting
• immunoblotting
• dot blot
• immunocytochemistry

生物化学的／生理学的作用

β-1 tubulin, also known as class VI or TUBB1, plays a vital role in platelet production and is considered to be a potential biomarker candidate for immune thrombocytopenia (ITP). Mutation in the gene leads to various neuronal migration disorders such as lissencephaly, pachygyria and polymicrogyria malformations. B2702 peptide binds to β-tubulin and inhibits natural killer (NK) cell cytotoxicity and it influences microtubule polymerization, which damages cytoskeleton organization and chaperone-like activity of tubulin. Tubulin β 2B class IIb (TUBB2B)/β-tubulin II plays a vital role in migration/organization and axon guidance processes. Mutation in β-tubulin gene is associated with the development of asymmetric polymicrogyria (PMG).

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable