T5951

抗TSG101(C末端)抗体 ウサギ宿主抗体

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• 別名: 抗腫瘍感受性遺伝子101抗体
• MDL番号: MFCD08705529
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: buffered aqueous solution
• 分子量: antigen 46 kDa (additional band of ~105 kDa may be observed in some preparations)
• 化学種の反応性: mouse, rat, human
• 濃度: ~1.5 mg/mL
• テクニック: western blot: 0.4-0.8 μg/mL using rat brain extract (S1 cytosolic fraction); western blot: 1-2 μg/mL using cell lysates of mouse fibroblast NIH3T3 cells and human A431 cells
• UniProtアクセッション番号: Q99816
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... TSG101(7251) ; mouse ... Tsg101(22088) ; rat ... Tsg101(292925)

詳細

詳細

Tumor susceptibility gene 101 encodes a multi-domain binding protein TSG101 which is 46 kDa. It is expressed in all tissues throughout development and is essential for both embryonic development and normal cell growth. It has an N-terminal UEV (N-terminal ubiquitin E2 variant) domain. Subcellular localization of TSG101 fluctuates in a cell-cycle dependent manner being localized in the nucleus and Golgi complex during interphase and in mitotic spindles and centrosome during mitosis.

免疫原

ヒトTSG101のC末端に位置するアミノ酸371~390に相当する合成ペプチドのKLH結合体。この配列は、ラット・マウスのTSG101と一致しています。

アプリケーション

Anti-TSG101 (C-terminal) antibody has been used in immnunoblotting and western blotting.

生物化学的／生理学的作用

TSG101 mediates a variety of cellular functions including transcriptional regulation, ubiquitination, endosomal trafficking, proliferation, and cell survival. It has a central role in protein sorting during endocytosis. Human TSG101 is also required for the budding of several enveloped RNA viruses including human immunodeficiency (HIV) and Ebola viruses. TSG101 is involved in the regulation of the p53/MDM2 (proto-oncogene) pathway by affecting E3 ubiquitin-protein ligase MDM2 (MDM2) stability. The N-terminal UEV domain binds to a P(T/S)AP sequence of HIV-1 p6 Gag and Ebola Vp40 proteins required for budding. The proline-rich domain was found to act as an activation domain in transcriptional regulation. The C-terminal coiled-coil (CC2) domain was predicted to interact with the cell growth regulating protein stathmin and is involved in potential co-repressor activity.

物理的形状

0.01M PBS溶液(pH 7.4, 15mMアジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)