T3320

Anti-γ-Tubulin Antibody

rabbit polyclonal

• MDL番号: MFCD00677366
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 製品名: 抗γ-チューブリン (AK-15) ウサギ宿主抗体, IgG fraction of antiserum, buffered aqueous solution
• 由来生物: rabbit
• 結合体: unconjugated
• 抗体製品の状態: IgG fraction of antiserum
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous solution
• 分子量: antigen 48 kDa
• 交差性: human, frog, rat
• 強化検証: independent; Learn more about Antibody Enhanced Validation
• テクニック: immunocytochemistry: 1:1,000 using methanol-acetone fixed, cultured rat, RAT-2 cells; microarray: suitable; western blot: 1:1,000 using whole cell extract of the human epitheloid carcinoma HeLa cell line.; western blot: 1:5,000 using Xenopus eggs extract
• UniProtアクセッション番号: P23258
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... TUBG1(7283) , TUBG2(27175) ; rat ... Tubg1(252921) , Tubg2(680991)

詳細

詳細

γ-Tubulin (48 kDa) is a highly conserved protein. The microtubule cytoskeleton consists of a dynamic, highly polarized network of microtubules filaments, microtubule-associated proteins, microtubule motors, and microtubule-organizing proteins. γ-Tubulin is found as part of a large protein complex containing at least five other proteins and has a shape of a ring (γ-tubulin ring complex, γ-TuRC) that is roughly the same diameter as a microtubule.

γ-Tubulin, mapped on human chromosome 17q21.2, codes for a member of the tubulin family. It is localized to the microtubule organizing centres. γ -Tubulin consists of two isoforms tubulinγ 1 (TUBG1) and tubulin γ 2 (TUBG2) with 97.3% amino acid identity. In addition to these two isoforms, γ-tubulin pseudogene (TUBG1P) is also been identified on human chromosome 17. Human TUBG1 and TUBG2 transcripts are widely expressed in preimplantation embryos and the brain, respectively. γ -tubulin is a component of γ-tubulin ring complex (γ-TuRC), which has roughly the same diameter as a microtubule.

アプリケーション

Anti-γ-Tubulin (AK-15) antibody produced in rabbit has been used in following studies:

• Western blot.
• Immunofluorescence.

Anti-γ-Tubulin (AK-15) antibody produced in rabbit has been used in:

• western blotting
• immunocytochemistry (immunofluorescence staining
• immunofluorescence

生物化学的／生理学的作用

γ-Tubulin nucleates microtubule assembly throughout the mammalian cell cycle in vivo. In Aspergillus nidulans, γ-tubulin facilitates attachment of microtubules to the spindle pole body, nuclear division and microtubule assembly. Ubiquitination of γ-tubulin by breast cancer 1 protein (BRCA1) is a crucial step in the regulation of centrosome number. Overexpression ofγ-tubulin is observed in lung cancer. Tubulin γ 2 (TUBG2) plays a vital role in cell growth. Aberration in the γ-tubulin gene alters microtubule assembly. The expression levels of γ-tubulin can be considered as an important prognostic indicator for patients with astrocytomas.

物理的形状

0.01M PBS溶液 (pH 7.4, 15mMアジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable