T1827

モノクロナール抗TBP マウス宿主抗体

clone 58C9, purified from hybridoma cell culture

• 別名: 抗GTF2DI抗体, 抗GTF2D抗体, 抗SCA17抗体, 抗TATAボックス結合タンパク質抗体, 抗TFIID抗体
• MDL番号: MFCD01322691
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 結合体: unconjugated
• 抗体製品の状態: purified from hybridoma cell culture
• 抗体製品タイプ: primary antibodies
• クローン: 58C9
• 分子量: antigen ~42 kDa
• 化学種の反応性: Sf9 cell line, human, yeast, Drosophila melanogaster
• テクニック: immunoprecipitation (IP): suitable; western blot: 1-2 μg/mL using nuclear extracts of D.Mel cells
• アイソタイプ: IgG2b
• UniProtアクセッション番号: P20226
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: Drosophila melanogaster ... Tbp(37476) ; human ... TBP(6908)

詳細

詳細

Monoclonal Anti-TBP (mouse IgG2b isotype) is derived from the hybridoma 58C9 produced by the fusion of mouse myeloma cells and splenocytes from Swiss Webster mice immunized with Drosophila TFIID complex. Transcription factor IID (TFIID) complex contains the TATA-binding protein (TBP) and over a dozen of TBP-associated factors (TAFs).

TBP (TATA-binding protein) is encoded by the gene mapped to human chromosome 6q27. The encoded protein is a key constituent of RNA polymerase II transcription apparatus in eukaryotic cells.

免疫原

Drosophila TFIID複合体。

アプリケーション

Monoclonal Anti-TBP antibody produced in mouse has been used in:

• immunoblotting
• co-immunoprecipitation
• immunohistochemistry

生物化学的／生理学的作用

TBP facilitates promoter recognition with the help of the sequence-specific binding of the TATA element found in many promoters.

The TBP (TATA-binding protein) gene encodes a subunit of TFIIIB (transcription factor IIIB) complex and recognizes the TATA box in the DNA. Thus, it plays an important role in RNA polymerase II transcription machinery. TBP is known to induce stress granule formation in hepatitis B viral infection. Mutation in the gene is associated with the development of spinocerebellar ataxia 17 (SCA17).

ターゲットの説明

TBP encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of tr

物理的形状

0.01 M PBS溶液 (pH 7.4, 15 mM アジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)