T1192

抗チミンダイマー抗体, マウスモノクローナル

clone H3, purified from hybridoma cell culture

• 別名: Mouse Anti-Thymine Dimer, Thymine Dimer Detection, Thymine Dimer Mouse Antibody
• MDL番号: MFCD01865339
• UNSPSCコード: 12352203
• NACRES: NA.41
• 結合体: unconjugated
• application: DB; ELISA (c); ICC
• クローン: H3, monoclonal
• 化学種の反応性: chicken, wide range
• citations: 7
• テクニック: capture ELISA: suitable; dot blot: 0.5-1 μg/mL; immunocytochemistry: suitable

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: H3, monoclonal
• フォーム: buffered aqueous solution
• 化学種の反応性: chicken, wide range
• 包装: antibody small pack of 25 μL
• 濃度: ~2 mg/mL
• テクニック: capture ELISA: suitable; dot blot: 0.5-1 μg/mL; immunocytochemistry: suitable
• アイソタイプ: IgG1
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

UV light induces the formation of thymine dimers that subsequently leads to DNA damage in cells. Monoclonal antibodies that bind to thymine dimers enable the study of cell death and UV-induced DNA damage during photocarcinogenesis . Monoclonal Anti-Thymine Dimer binds to the (5′-6′) cyclobutane type of homothymine or thyminecytosine heterodimers in single-stranded DNA. The antibody has a decreased affinity for the dimer in short oligonucleotides (an oligonucleotide of atleast 10-20 thymine residues is required for the labeling of the probes). The product facilitates the use of a sensitive and non-radioactive technique for the analysis of thymine dimerization.

免疫原

シクロブタンチミンダイマ-を含有する4個のヌクレオチド(GTTG)の、ニワトリγグロブリン結合体す。

アプリケーション

May be used in Southern blotting at 0.5-1μg/mL using UV induced thymine dimers of λ phage DNA fragments.

Monoclonal Anti-Thymine Dimer antibody is suitable for use in dot blot (0.5-1 mg/mL), immunocytochemistry (1:100) and capture ELISA.

物理的形状

0.01M PBS溶液(pH 7.4, 15mMアジ化ナトリウム含有)。

その他情報

Distributed under license.
Patents WO87/01134, EP 0233 177 B1

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable