SRP4760

ApoE2 ヒト

recombinant, expressed in E. coli, ≥90% (SDS-PAGE), ≥90% (HPLC)

• 別名: アポリポタンパク質E2
• CAS番号: 456542-91-9
• MDL番号: MFCD00285513
• UNSPSCコード: 12352202
• NACRES: NA.32

特性

• 由来生物: human
• リコンビナント: expressed in E. coli
• アッセイ: ≥90% (HPLC); ≥90% (SDS-PAGE)
• 形状: lyophilized
• 分子量: ~34 kDa
• 包装: pkg of 500 μg
• 保管条件: avoid repeated freeze/thaw cycles
• 不純物: endotoxin, tested
• NCBIアクセッション番号: NM_000041
• UniProtアクセッション番号: P02649
• 輸送温度: wet ice
• 保管温度: −20°C
• 遺伝子情報: human ... ApoE(348)

詳細

詳細

Apolipoprotein E (ApoE) belongs to a group of proteins that bind reversibly with lipoproteins. Significant quantities of ApoE are produced in liver and brain and to some extent in almost every organ. ApoE is an important constituent of all plasma lipoproteins. ApoE exists in three major isoforms; E2, E3, and E4, which differ from one another by a single amino-acid substitution. Compared with E3 and E4, E2 exhibits the lowest receptor binding affinity. E2 allele carriers have significantly lower levels of total cholesterol, low-density lipoprotein cholesterol, and non-high-density lipoprotein cholesterol, as well as increased ApoE levels. The gene encoding this protein is localized on human chromosome 19q13.32. Recombinant human ApoE2 is a 34.3kDa protein containing 300 amino acid residues.

生物化学的／生理学的作用

In addition to facilitating solubilization of lipids, apolipoproteins help to maintain the structural integrity of lipoproteins, serve as ligands for lipoprotein receptors, and regulate the activity of enzymes involved in lipid metabolism. Apolipoprotein E (ApoE) plays an important role in lipid metabolism. It′s interaction with specific ApoE receptor enables uptake of chylomicron remnants by liver cells, which is an essential step during normal lipid metabolism. It also binds with the LDL receptor (Apo B/E). Defects in ApoE are a cause of hyperlipoproteinemia type III.

物理的形状

凍結乾燥品(ろ過滅菌済み、添加剤不含)。

調製ノート

バイアルは、開封前に遠心してください。凍結融解の繰り返しは避けてください。

再構成

dH₂Oで濃度0.1～1.0 mg/mLに再構成してください。溶液は他の水溶性バッファーで希釈することが可能であり、4°Cで1週間、-20°Cではさらに保存することができます。

免責事項

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable