SRP3097

IL-6 レセプター ヒト

recombinant, expressed in CHO cells, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

• 別名: B細胞刺激因子-2, CD126, 可溶性 IL-6 レセプターα
• UNSPSCコード: 12352202
• NACRES: NA.32

特性

• 由来生物: human
• リコンビナント: expressed in CHO cells
• アッセイ: ≥95% (HPLC); ≥95% (SDS-PAGE)
• フォーム: lyophilized
• 有効性: ≤5.0 ng/mL ED₅₀
• 分子量: 37.9 kDa
• 包装: pkg of 20 μg
• テクニック: cell culture | mammalian: suitable
• 不純物: <1 EU/μg endotoxin, tested
• 色: white to off-white
• 適合性: suitable for molecular biology
• UniProtアクセッション番号: P08887
• 輸送温度: wet ice
• 保管温度: −20°C
• 遺伝子情報: human ... IL6R(3570)

詳細

詳細

Interleukin-6 (IL-6) mediates its biological effects through the type I IL-6 receptor system that consists of two chains, IL-6Rα and gp130. The IL-6Rα chain is the binding component specific to IL-6; while the gp130 only transmits signals of IL-6 when bound to IL-6Rα. IL-6R has a role in various signaling cascades. The gp130 also can transmit signals from leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor (CNTF), interleukin-11 (IL-11) and cardiotrophin-1 (CT-1) in conjunction with other receptor subunits. The low-affinity binding site for IL-6 is composed of IL-6Rα alone. IL-6Rα is expressed in a wide range of cells including T cells, fibroblasts and macrophages. Soluble IL-6Rα which consists of only the extracellular domain of the IL-6Rα chain, acts as an agonist of IL-6 activity at low concentrations. Recombinant human sIL-6Rα is a 37.6kDa protein consisting of the extracellular domain of the IL-6Rα chain (339 amino acid residues).

Research Area: IMMUNO AND CKS
Interleukin-6 Receptor Alpha (IL-6Rα) is a transmembrane protein.

アプリケーション

sIL-6 Receptor alpha human has been used in cell culture to treat fibroblasts. It has also been used in stimulation experiments to analyze IL-6 trans-signaling.

生物化学的／生理学的作用

Interleukin-6 Receptor Alpha (IL-6Rα) plays a dual role in signaling. In classical signaling, it interacts with glycoprotein 130 to promote the anti-inflammatory and regenerative effects of IL-6. In contrast, during trans-signaling, it facilitates the pro-inflammatory effects of IL-6 by allowing cells lacking IL-6Rα to respond to IL-6 in the presence of soluble IL-6Rα. IL-6Rα exists in both soluble and transmembrane forms. The transmembrane form is integrated into the cell membrane of target cells. IL-6 and the IL-6 receptor alpha chain (IL-6R) are expressed by various cell types, including monocytes, lymphocytes, fibroblasts, and endothelial cells.

シーケンス

LAPRRCPAQE VARGVLTSLP GDSVTLTCPG VEPEDNATVH WVLRKPAAGS HPSRWAGMGR RLLLRSVQLH DSGNYSCYRA GRPAGTVHLL VDVPPEEPQL SCFRKSPLSN VVCEWGPRST PSLTTKAVLL VRKFQNSPAE DFQEPCQYSQ ESQKFSCQLA VPEGDSSFYI VSMCVASSVG SKFSKTQTFQ GCGILQPDPP ANITVTAVAR NPRWLSVTWQ DPHSWNSSFY RLRFELRYRA ERSKTFTTWM VKDLQHHCVI HDAWSGLRHV VQLRAQEEFG QGEWSEWSPE AMGTPWTESR SPPAENEVST PMQALTTNKD DDNILFRDSA NATSLPVQD

物理的形状

Lyophilized powder.

再構成

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

安全性情報

• 保管分類コード: 13 - Non Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable