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リコンビナント
expressed in baculovirus
アッセイ
≥80% (SDS-PAGE)
フォーム
aqueous solution
分子量
46.9 kDa
NCBIアクセッション番号
輸送温度
dry ice
保管温度
−70°C
遺伝子情報
human ... SPHK1(8877)
詳細
Human Sphingosine kinase 1 (GenBank Accession No. AF200328), full length with N-terminal His tag, MW = 46.9 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
アプリケーション
酵素キネティクスの研究をはじめ、インヒビターのスクリーニングや選択性プロファイリングにもご利用いただけます。
単位の定義
One unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P/min
物理的形状
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.
調製ノート
氷上で解凍してください。最初に解凍した際、チューブを軽く遠心して内容物を完全に回収してください。酵素液は一回の使用量ずつ分注してください。分注された希釈前の酵素を-70°Cで保存してください。注記:酵素は凍結解凍の繰り返しに非常に弱いので、注意してください。
最新バージョンのいずれかを選択してください：
試験成績書（COA）
Lot/Batch Number
Deborah A Pasternack et al.
mBio, 6(5), e01291-e01215 (2015-10-08)
Sphingolipids are important constituents of cell membranes and also serve as mediators of cell signaling and cell recognition. Sphingolipid metabolites such as sphingosine-1-phosphate and ceramide regulate signaling cascades involved in cell proliferation and differentiation, autophagy, inflammation, and apoptosis. Little is
Sphingosine kinase 1 in viral infections
Carr, J., et al.
Reviews in Medical Virology, DOI: 10-DOI: 10 (2012)
Shi-Quan Liu et al.
International journal of colorectal disease, 27(12), 1569-1578 (2012-06-12)
Sphingosine kinase (SphK) 1 is an oncogenic enzyme promoting transformation, proliferation, and survival of a number of human tumor cells. However, its effect on colon cancer cell behavior has not been fully clarified. SphK1 plasmid or SphK1 shRNA transfection and
Jian Pan et al.
Journal of translational medicine, 9, 157-157 (2011-09-23)
Treatment failure for esophageal carcinoma is frequently due to lymph node metastasis and invasion to neighboring organs. The aim of the present study was to investigate invasion- and metastasis-related genes in esophageal carcinoma cells in vitro and in vivo. A
Francesca Bini et al.
Neuropharmacology, 63(4), 524-537 (2012-05-15)
1α,25-Dihydroxyvitamin D3 (1,25(OH)₂D₃), a crucial regulator of calcium/phosphorus homeostasis, has important physiological effects on growth and differentiation in a variety of malignant and non-malignant cells. Synthetic structural hormone analogues, with lower hypercalcemic side effects, are currently under clinical investigation. Sphingolipids
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