SRP0166

DNMT3B/DNMT3L Active human

recombinant, expressed in baculovirus infected insect cells, ≥90% (SDS-PAGE)

• 別名: DNA (cytosine-5-)-methyltransferase 3b, ICF, M.HsaIIIB
• UNSPSCコード: 12352200
• NACRES: NA.32

特性

• 由来生物: human
• リコンビナント: expressed in baculovirus infected insect cells
• アッセイ: ≥90% (SDS-PAGE)
• フォーム: aqueous solution
• 分子量: 27 kDa (DNMT3L); 59 kDa (DNMT3B)
• 包装: pkg of 10 μg
• 濃度: >0.02 mg/mL
• NCBIアクセッション番号: NM_006892 (DNMT3B); NM_013369 (DNMT3L)
• UniProtアクセッション番号: Q9UBC3
• 輸送温度: dry ice
• 保管温度: −70°C
• 遺伝子情報: human ... DNMT3B(1789)

詳細

詳細

DNA (cytosine-5-)-methyltransferase 3 β (DNMT3B) has two subfamilies-DNMT3B and ΔDNMT3B. These are expressed in many transcript variants. Several single-nucleotide polymorphisms are observed within the promoter region of the DNMT3B gene. The gene encoding this protein is localized on human chromosome 20q11.2. DNA (cytosine-5)-methyltransferase 3-like (DNMT3L) is part of the DNA methyltransferases family. It has a cysteine-rich region containing a novel-type zinc finger domain. The gene encoding it is localized on human chromosome 21q22.3.

アプリケーション

Useful for the study of DNA methylation, screening inhibitors, and selectivity profiling.

生物化学的／生理学的作用

DNA (cytosine-5-)-methyltransferase 3 β (DNMT3B) is a de novo methyltransferase which targets the un-methylated 5′-C-phosphate-G-3′ (CpG) sites on the gene promoter. It is responsible for crucial epigenetic mechanisms for the regulation of chromosomal stability and for building a new methylation pattern. The protein is associated with promoter hypermethylation in tumorigenesis and lung cancer. DNA (cytosine-5)-methyltransferase 3-like (DNMT3L) is a non-catalytic accessory factor which is mainly responsible for the stimulation of DNA methylation. It helps the DNA methylation machinery to be positioned on properly chromatinized DNA regions. The protein also helps to provide uniform methylation patterns. It has an important role in spermatogenesis and mutations in the DNMT3L gene might be associated with azoospermia susceptibility.

物理的形状

処方:40 mM Tris-HCl、pH 8.0、110 mM NaCl、2.2 mM KCL、20%グリセロール、4 mMグルタチオン。

調製ノート

氷上で解凍してください。最初に解凍した際、チューブを軽く遠心して内容物を完全に回収してください。酵素液は一回の使用量ずつ分注してください。分注した酵素液は希釈せずそのまま-70°Cで保存してください。注:酵素は凍結融解の繰り返しに対して非常に不安定です。

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable