SBR00023

1-Cyano-4-dimethylaminopyridinium tetrafluoroborate Ready Made Solution

organic cyanylating reagent, 100 mg/mL in acetonitrile

• 別名: 4-(dimethylamino)pyridin-1-ium-1-carbonitrile tetrafluoroborate, CDAP
• 実験式（ヒル表記法）: C₈H₁₀BF₄N₃
• CAS番号: 59016-56-7
• 分子量: 234.99
• UNSPSCコード: 12161902
• NACRES: NA.28

特性

• 由来生物: synthetic
• アッセイ: ≥97%
• 形状: liquid
• 分子量: 234.99
• 濃度: 100 mg/mL in acetonitrile
• 輸送温度: dry ice
• 保管温度: −20°C
• InChI: 1S/C8H10N3.BF4/c1-10(2)8-3-5-11(7-9)6-4-8;2-1(3,4)5/h3-6H,1-2H3;/q+1;-1
• InChI Key: MBLVMDCQDCVKNE-UHFFFAOYSA-N

詳細

詳細

1-Cyano-4-dimethylaminopyridinium tetrafluoroborate (CDAP) is crucial in vaccine and biochemical research. It rapidly activates polysaccharides, like TI-2 antigens, in a short time. Further, CDAP enables protein conjugation, vital for creating conjugate vaccines that enhance immune responses. CDAP works effectively at both pH 9-10 and mild alkaline conditions (pH 7-9), making it versatile for various antigens. CDAP′s efficacy in inducing high antibody levels makes it invaluable for developing effective vaccines and immunological reagents.

アプリケーション

1-Cyano-4-dimethylamino pyridinium tetrafluoroborate can be used in vaccine research for the conjugation of polysaccharides with proteins

生物化学的／生理学的作用

CDAP is a cyanylation reagent for protein sulfhydryl groups which is used to prepare protein-polysaccharide conjugates. CDAP is also an activating agent for polysaccharide resins and is used for the conjugation of lipopolysaccharides while retaining their endotoxic activity.

CDAP is considered to be a less toxic reagent as compared to cyanogen bromide (CNBr) (a known polysaccharides activator). In addition, CDAP is easier to use as it can be employed at a lower pH and has fewer side reactions. It is known that CDAP polysaccharide activation efficiency is optimal at pH 9-10. It was also reported that direct conjugation of protein to CDAP-activated polysaccharides can be performed under mildly alkaline conditions (pH 7-9). It has also been reported that proteins could also be conjugated to CDAP-activated polysaccharides at pH 5.

特徴および利点

• Readily available solution, that reduces the need for preparation time
• Versatile and adaptable for vaccine and biochemical research

調製ノート

Polysaccharide conjugation: CDAP Ready Made Solution (100mg/mL in acetonitrile) should be added in a ratio of 1mg CDAP to 1mg polysaccharide while vortexing (Example: per 1mg polysaccharide add 10μL of CDAP Ready Made Solution). Add 0.2M triethylamine, 10μL per 1mg polysaccharide to raise the pH of the reaction.

その他情報

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安全性情報

• ピクトグラム: GHS02,GHS07
• シグナルワード: Danger
• 危険有害性情報: H225,H302 + H312 + H332,H315,H319
• 注意書き: P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P312 - P305 + P351 + P338
• 危険有害性の分類: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2
• 保管分類コード: 3 - Flammable liquids
• WGK: WGK 3
• 引火点(°F): 35.6 °F
• 引火点(℃): 2 °C