SAE0215

Inorganic Pyrophosphatase from Escherichia coli

≥100 units/mL, buffered aqueous solution

• UNSPSCコード: 12352204

特性

• 由来生物: Escherichia coli
• 品質水準: 200
• リコンビナント: expressed in E. coli
• グレード: for molecular biology
• 詳細: Recombinant, expressed in E.coli
• アッセイ: ≥95% (size exclusion chromatography)
• フォーム: buffered aqueous solution
• 比活性: ≥100 units/mL
• シェルフライフ: 2 yr at -20 °C ((retest))
• 分子量: 19.7 kDa
• 保管条件: OK to freeze
• 濃度: ≥100 units/mL
• 色: colorless
• 最適pH: 9.0 (25 °C)
• pH: 8.0 (25 °C)
• 溶解性: soluble; water: miscible
• 適合性: suitable for molecular biology
• UniProtアクセッション番号: P0A7A9
• アプリケーション: research use
• その他の活性: DNAse, none detected; RNAse, none detected; Nickase, none detected
• 輸送温度: dry ice
• 保管温度: -10 to -25°C

詳細

詳細

Inorganic pyrophosphatase (PPase) is a ubiquitous enzyme that catalyzes pyrophosphate hydrolysis. It plays an important role in energy metabolism by providing a thermodynamic pull for biosynthetic reactions, such as protein, RNA, and DNA synthesis. Nucleic acid synthesis would be energetically impossible in vivo if not coupled with the hydrolysis of pyrophosphate (PPi).

アプリケーション

This product is based on the native pyrophosphatase from E. coli, Uniprot No. P0A7A9. Pyrophosphatase in E. coli is a homohexameric protein containing 175 amino acids residues per subunit. This product is a recombinant protein expressed in E. coli and induced by IPTG. Each subunit has a MW of 19.7 kDa and theoretical pI of ~5. The protein activity is Mg2+ dependent and it is a relatively thermostable protein.

生物化学的／生理学的作用

Inorganic pyrophosphatase (PPase) is a ubiquitous enzyme that catalyzes pyrophosphate to phosphate. It plays an important role in energy metabolism as it provides a thermodynamic pull for biosynthetic reactions, such as protein, RNA, and DNA synthesis.

特徴および利点

This product has a purity minimum of 95% (SEC-HPLC) and an activity minimum of 100 units/mL to enhance RNA yield during transcription.

単位の定義

One unit will release 1.0 µmole of inorganic orthophosphate per minute at pH 9 at 25 °C. The reaction buffer used for determination of enzyme activity contains 50 mM Tris-HCl, pH 9.0.

物理的形状

The product is supplied as an aqueous solution containing 20mM Tris-HCl, 100mM NaCl, 1mM DTT, 0.1mM EDTA, and 50% glycerol, titrated to pH 8 at 25 °C.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1