SAB4200853

Anti-PBP2a of MRSA antibody, Mouse monoclonal

clone 38, purified from hybridoma cell culture

• 別名: Anti-Beta-lactam-inducible penicillin-binding protein 2a
• UNSPSCコード: 12352203
• NACRES: NA.43

特性

• 抗体製品の状態: purified from hybridoma cell culture
• 品質水準: 200
• 抗体製品タイプ: primary antibodies
• クローン: 38, monoclonal
• フォーム: liquid
• 濃度: ~1 mg/mL
• テクニック: immunoblotting: 0.5-1 μg/mL using whole recombinant PBP2a protein
• アイソタイプ: IgG1
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

Infections caused by methicillin-resistant Staphylococcus aureus (MRSA) are a major concern cause for clinicians, presenting high mortality and morbidity rates compared to infections caused by methicillin-sensitive Staphylococci.2

特異性

Monoclonal Anti-PBP2a of MRSA antibody specifically recognizes PBP2a protein from Methicillin-resistant Staphylococcus aureus (MRSA). The antibody reacts with both denaturated recombinant PBP2a protein and bacteria lysates in Immunoblot.1 The antibody recognizes PBP2a in MRSA cells in both the exponential and stationary phases, however no cross reaction with methicillin-sensitive Staphylococcus aureus (PBP2a negative) and E. coli were seen.1

アプリケーション

The antibody may be used in various immunochemical techniques including Immunoblotting1 (~76 kDa), ELISA1, Surface Plasmon Resonance (SPR)1 and Flow cytometry1.

生物化学的／生理学的作用

Resistance of S. aureus to methicillin and all beta-lactam antibiotics is due to the activity of PBP2a protein (i.e., penicillin binding protein2a) that is located in the cell wall of the resistant strains.3-4 PBP2a is encoded by mecA gene and belongs to a family of penicillin binding proteins (PBPs). PBPs are membrane-bound enzymes that catalyze the transpeptidation reaction necessary for cross-linkage of peptidoglycan chains.5-6 Contrary to all PBPs, PBP2a has low affinity for all ß-lactam antibiotics.7 Therefore, in the presence of ß-lactam antibiotics, while normal PBPs are blocked, the PBP2a is able to continue catalyze the transpeptidation reactions enabling cell wall synthesis.4 PBP2a protein is suggested to be a promising candidate for anti-MRSA vaccine development.8

物理的形状

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.Antibody Concentration: ~ 1.0 mg/mL

保管および安定性

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免責事項

Unless otherwise stated in our catalog  our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable