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由来生物
goat
品質水準
結合体
phycoerythrin (R-PE) conjugate
抗体製品の状態
affinity isolated antibody
抗体製品タイプ
secondary antibodies
クローン
polyclonal
形状
buffered aqueous solution
テクニック
direct immunofluorescence: 1:16
輸送温度
wet ice
保管温度
2-8°C
ターゲットの翻訳後修飾
unmodified
詳細
Affinity isolated antigen specific antibody is obtained from goat anti-human IgG antiserum by immunospecific purification which removes essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the γ-chain of human IgG. Goat anti-human IgG is conjugated to Sigma R-Phycoerythrin (Product No. P8912) by a modification of the method of Kronick Unbound phycoerythrin is removed by gel filtration chromatography
Specificity for the γ-chain of human IgG is determined by Ouchterlony Double Diffusion (ODD). The antibody preparation is specific for human IgG when tested against purified human IgA, IgG, IgM, Bence Jones κ and Bence Jones Lambda myeloma proteins, prior to conjugation
Identity and purity of the antibody is established by immunoelectrophoresis (IEP), prior to conjugation Electrophoresis of the antibody preparation followed by diffusion versus anti-goat IgG and anti-goat whole serum results in single arcs of precipitation.
Specificity for the γ-chain of human IgG is determined by Ouchterlony Double Diffusion (ODD). The antibody preparation is specific for human IgG when tested against purified human IgA, IgG, IgM, Bence Jones κ and Bence Jones Lambda myeloma proteins, prior to conjugation
Identity and purity of the antibody is established by immunoelectrophoresis (IEP), prior to conjugation Electrophoresis of the antibody preparation followed by diffusion versus anti-goat IgG and anti-goat whole serum results in single arcs of precipitation.
Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders
Anti-Human IgG (γ-chain specific)-R-Phycoerythrin antibody is specific for human IgG when tested against purified human IgA, IgG, IgM, and Bence Jones κ and λ myeloma proteins.
Anti-Human IgG (γ-chain specific)-R-Phycoerythrin antibody is specific for human IgG when tested against purified human IgA, IgG, IgM, and Bence Jones κ and λ myeloma proteins.
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. It consists of a gamma (γ) heavy chain in the constant (C) region. The primary structure of the antibody contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and also resides inside the chains.
免疫原
Developed in goat using purified human IgG as the immunogen.
Purified human IgG
アプリケーション
A minimum 1:16 dilution is required for direct immunofluorescent labelling of human peripheral blood lymphocytes. The antibody may be used in fluorescence-activated cell sorting applications.
物理的形状
0.01 M PBS溶液(pH 7.4, 0.1 mM EDTA, 1 mMヨ-ドアセトアミド, 1% BSA, 15 mMアジ化ナトリウム含有)
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
P9170-.25ML:
P9170-.5ML:
P9170-BULK:
P9170PROC:
P9170-VAR:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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OPP Labeling Enables Total Protein Synthesis
Quantification in CHO Production Cell Lines at the
Single-Cell Level
Quantification in CHO Production Cell Lines at the
Single-Cell Level
Nagelreiter F, et al.
Biotechnology Journal, 13 (2018)
Soren U Nielsen et al.
Blood, 100(12), 4067-4073 (2002-10-24)
We have previously shown that bivalent human gamma1 CD3 monoclonal antibody (mAb) is ineffective at mediating lysis of human T cells with human complement. In this paper we have used genetic engineering and sulfur chemistry to prepare 2 types of
Jackson AL, NL Warner
Manual of Clinical Laboratory Immunology, 3 edition, 226-226 (1986)
Ingrid J G Burvenich et al.
mAbs, 8(4), 775-786 (2016-04-01)
IgG has a long half-life through engagement of its Fc region with the neonatal Fc receptor (FcRn). The FcRn binding site on IgG1 has been shown to contain I253 and H310 in the CH2 domain and H435 in the CH3
The use of phycobiliproteins as fluorescent labels in immunoassay.
M N Kronick
Journal of immunological methods, 92(1), 1-13 (1986-08-21)
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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