コンテンツへスキップ
Merck

SAB4200643

Sigma-Aldrich

Monoclonal Anti-PLEKHA7 antibody produced in mouse

clone PLK7, purified from hybridoma cell culture

ログイン組織・契約価格を表示する


About This Item

UNSPSCコード:
12352203
NACRES:
NA.41

由来生物

mouse

品質水準

抗体製品の状態

purified immunoglobulin

抗体製品タイプ

primary antibodies

クローン

PLK7, monoclonal

フォーム

buffered aqueous solution

交差性

human

濃度

~1 mg/mL

テクニック

flow cytometry: 2-4 μg/test using NCI-H1299 cells
immunoblotting: suitable
immunocytochemistry: 0.25-0.5 μg/mL using human CaCo2 cells

アイソタイプ

IgG2a

UniProtアクセッション番号

輸送温度

dry ice

保管温度

−20°C

ターゲットの翻訳後修飾

unmodified

遺伝子情報

詳細

Monoclonal Anti-PLEKHA7 (mouse IgG2a isotype) is derived from the hybridoma PLK7 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a sequence at the N-terminal region of human PLEKHA7, conjugated to KLH. PLEKHA7 contains WW (rsp5-domain), PH (pleckstrin homology), and CC (coiled-coil) domains.
PLEKHA7 (Pleckstrin homology domain containing, family A member 7) is an adherens junction protein localized at the adherens junctions in colonic epithelial cells.

免疫原

synthetic peptide corresponding to a sequence at the N-terminal region of human PLEKHA7

アプリケーション

Monoclonal Anti-PLEKHA7 antibody has been used in
  • immunoblotting
  • immunocytochemistry
  • flow cytometry

生物化学的/生理学的作用

Overexpression of PLEKHA7 was reported to be common in invasive lobular carcinomas (ILCs) and hence PLEKHA7 could serve as a molecular marker to differentiate ILCs from invasive ductal carcinomas (IDCs).
PLEKHA7 (Pleckstrin homology domain containing, family A member 7) functions as a cytoplasmic component of the epithelial adherens junction belt, with a subcellular localization and tissue distribution and connects E-cadherin-p120 ctn complex to the microtubule cytoskeleton. It has been reported that PLEKHA7 acts as zonula adherens (ZA) component, identifies Nezha, which further binds to microtubules (MTs) of the cytoskeleton, and forms a protein complex during cytoskeleton assembly.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

適切な製品が見つかりませんか。  

製品選択ツール.をお試しください

保管分類コード

10 - Combustible liquids

WGK

WGK 1

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

SAB4200643-VAR:
SAB4200643-BULK:
SAB4200643-100UL:


最新バージョンのいずれかを選択してください:

試験成績書(COA)

Lot/Batch Number

適切なバージョンが見つかりませんか。

特定のバージョンが必要な場合は、ロット番号またはバッチ番号で特定の証明書を検索できます。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Anna Akhmanova et al.
Cell, 135(5), 791-793 (2008-12-02)
The zonula adherens (ZA) is a specialized cadherin-based structure found at the contacts between epithelial cells. Meng et al. (2008) now identify a protein complex containing the microtubule minus-end-binding protein Nezha, which provides a critical link between microtubules and cadherins
Souichi Kurita et al.
The Journal of biological chemistry, 288(41), 29356-29368 (2013-08-31)
Adherens junction (AJ) is a specialized cell-cell junction structure that plays a role in mechanically connecting adjacent cells to resist strong contractile forces and to maintain tissue structure, particularly in the epithelium. AJ is mainly comprised of cell adhesion molecules
Bàrbara Castellana et al.
Human pathology, 43(11), 1902-1909 (2012-05-01)
Molecular differentiation between invasive lobular carcinomas (ILCs) and invasive ductal carcinomas (IDCs) of the breast has not been well defined. We investigated gene expression differences between ILCs and IDCs and their correlation with variations in invasiveness and tumor growth. Total
Pamela Pulimeno et al.
PloS one, 5(8), e12207-e12207 (2010-09-03)
The pleckstrin-homology-domain-containing protein PLEKHA7 was recently identified as a protein linking the E-cadherin-p120 ctn complex to the microtubule cytoskeleton. Here we characterize the expression, tissue distribution and subcellular localization of PLEKHA7 by immunoblotting, immunofluorescence microscopy, immunoelectron microscopy, and northern blotting
Wenxiang Meng et al.
Cell, 135(5), 948-959 (2008-12-02)
Epithelial cells contain noncentrosomal microtubules (MTs), whose minus ends are oriented apically. In contrast with the well-known interactions of the minus ends with the centrosome, little is known about the termination site of the noncentrosomal minus ends. Here we show

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

製品に関するお問い合わせはこちら(テクニカルサービス)