SAB4200552

Anti-WASH1 antibody, Mouse monoclonal

clone WASH1-27, purified from hybridoma cell culture

• 別名: Anti-CXYorf1-like protein on chromosome 9, Anti-FAM39E, Anti-WAS protein family homolog 1, Anti-WASH, Monoclonal Anti-WASH1 antibody produced in mouse
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 結合体: unconjugated
• 抗体製品の状態: purified from hybridoma cell culture
• 抗体製品タイプ: primary antibodies
• クローン: WASH1-27, monoclonal
• 形状: buffered aqueous solution
• 分子量: antigen ~72 kDa
• 化学種の反応性: mouse, human
• 濃度: ~1 mg/mL
• テクニック: immunoprecipitation (IP): 5-10 μg using lysates of human HeLa cells; western blot: 2.5-5.0 μg/mL using whole extracts of HEK-293T cells overexpressing mouse WASH1
• アイソタイプ: IgG1
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... WASH1(100287171)

詳細

詳細

Monoclonal Anti-WASH1 (mouse IgG1 isotype) is derived from the hybridoma WASH1-27 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to an internal region of human WASH1. WASH1 (Wiskott-Aldrich Syndrome Protein and SCAR Homolog), a new member of the Wiskott−Aldrich Syndrome protein (WASP) family. Similar to other WASP family members, it contains a C-terminal for ′WH2, connecting and acidic′ (WCA) domain that binds to actin and the Arp2/3 complex. In addition, WASH1 also contains a short proline-rich region, a unique N-terminal domain termed WASH-homology domain (WAHD1), and a tubulin-binding region. Through its WAHD1 region, it interacts with family with sequence similarity 21 (FAM21), a protein that links WASH1 to endosomes. WASH1 forms part of a multiprotein complex composed of FAM21, KIAA1033 strumpellin and WASH-interacting protein (SWIP) and coiled coil domain containing 53 (CCDC53).

免疫原

a synthetic peptide corresponding to an internal region of human WASH1 (GeneID: 100287171), conjugated to KLH. The corresponding sequence differs by one amino acid in mouse and rat.

アプリケーション

Monoclonal Anti-WASH1 antibody produced in mouse has been used in may be used in various immunochemical techniques including immunoblotting (~72 kDa), immunoprecipitation and immunofluorescence.

生物化学的／生理学的作用

WASH1 (Wiskott-Aldrich Syndrome Protein and SCAR Homolog), is a nucleation-promoting factor at the surface of endosomes. It recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of endosomes. It associates with tubulin and localizes to early and recycling endosomes, where together with the Arp2/3 complex and actin, it is required for maintaining the shape of the endosomal compartment and the regulation of the retrograde transport.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable