SAB4200489

Anti-Occludin (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• 別名: 抗OCLN抗体
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous solution
• 分子量: antigen ~65 kDa
• 化学種の反応性: human, dog
• 濃度: ~1.0 mg/mL
• テクニック: immunohistochemistry: 10-20 μg/mL using formalin-fixed, paraffin-embedded human colon.; indirect immunofluorescence: 1-2 μg/mL using MDCK cells; western blot: 1.5-3.0 μg/mL using extracts of Caco-2 cells
• UniProtアクセッション番号: Q16625
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... OCLN(100506658)

詳細

詳細

Occludin (OCLN) is an integral membrane protein, encoded by the gene mapped to human chromosome 5q13.2. OCLN has a molecular weight of about 65kDa. It possesses four transmembrane domains including a long COOH-terminal cytoplasmic domain, a short NH2-terminal cytoplasmic domain, two extracellular loops and one intracellular turn. It is specifically localized at tight junctions (TJ).

免疫原

a synthetic peptide corresponding to a sequence at the C-terminal region of human occludin, conjugated to KLH. The corresponding sequence is highly conserved in rat (89% sequence identity) and in mouse (84% sequence identity) occludin.

アプリケーション

Anti-Occludin (C-terminal) antibody produced in rabbit has been used in immunohistochemistry.

生物化学的／生理学的作用

Occludin (OCLN), along with tight junction (TJ)-associated peripheral membrane proteins plays a vital role in the formation and regulation of TJ. The encoded protein facilitates the translocation of p85a (regulatory subunit of phosphoinositide 3-kinase) to TJs to regulate actin organization after oxidative stress. OCLN is also implicated in cell migration. Mutation in the gene leads to the development of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG).

物理的形状

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable