S5569

Anti-Superoxide Dismutase (MnSOD) (KC-19) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• 別名: 抗IPO-B抗体, 抗MNSOD抗体, 抗SOD2抗体, 抗インドフェノールオキシダーゼB抗体, 抗スーパーオキシドジスムターゼ2抗体, 抗マンガンスーパーオキシドジスムターゼ2抗体
• MDL番号: MFCD06798546
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: IgG fraction of antiserum
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous solution
• 分子量: antigen ~24 kDa
• 化学種の反応性: rat, human
• 強化検証: independent; Learn more about Antibody Enhanced Validation
• テクニック: indirect immunofluorescence: 1:250-1:500 using methanol-acetone-fixed rat NRK cells; western blot: 1:1,000-1:2,000 using whole extract of rat brain and a chemiluminescent detection reagent; western blot: 1:2,000-1:4,000 using human HeLa mitochondria extract and a chemiluminescent detection reagent
• UniProtアクセッション番号: P04179
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... SOD2(6648) ; mouse ... Sod2(20656) ; rat ... Sod2(24787)

詳細

詳細

Manganese superoxide dismutase (MnSOD) protein is a homotetrameric SOD. This protein is composed of four subunits, each containing one Mn²⁺. MnSOD enzyme is synthesized in the cytosol and is modified for its transport into the mitochondria where it resides in the matrix.

The SOD2 (superoxide dismutase 2) gene is mapped to human chromosome 6q25.3. The encoded protein functions as a tetramer and is localized to mitochondria.

特異性

Anti-Superoxide Dismutase (MnSOD) (KC-19) recognizes human and rat Superoxide Dismutase (MnSOD).

免疫原

synthetic peptide corresponding to amino acid residues 202-220 of human superoxide dismutase (MnSOD), conjugated to KLH. The corresponding sequence is identical in apes and monkeys, differs by one amino acid in mouse and horse, by two amino acids in cow, and by four amino acids in rat.

アプリケーション

Anti-Superoxide Dismutase (MnSOD) (KC-19) antibody produced in rabbit may be used in immunoblotting and indirect immunofluorescence.

生物化学的／生理学的作用

Manganese superoxide dismutase (MnSOD) is essential for the survival of aerobic life. This protein is inducible by tumor necrosis factor (TNF) and protects cells from TNF-mediated apoptosis. MnSOD is proposed to function as a tumor suppressor by modulating the activity of redox-sensitive transcription factors and specific signal mediators. Overexpression of the MnSOD gene protects neurons from N-methyl-D-aspartate receptor (NMDA) and nitric oxide-induced neurotoxicity. A decline in the MnSOD activity occurs in aging, progeria, cancer, asthma and transplant rejection.

Superoxide Dismutase 2 (SOD2) is an antioxidant enzyme and catalyzes the conversion of superoxide to hydrogen peroxide and molecular oxygen. The enzyme offers protection against oxidative damage induced by reactive oxygen species and also is radioprotective. The gene is associated with the metastasis and invasion process of salivary adenoid cystic carcinoma. Manganese SOD is involved in detoxification.

物理的形状

PBS溶液 (pH 7.4, 15mMアジ化ナトリウム含有)。

保管および安定性

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable