S4945

抗SynCAM ウサギ宿主抗体

affinity isolated antibody, buffered aqueous solution

• 別名: 抗IGSF4抗体, 抗Necl-2抗体, 抗RAI75抗体, 抗SgIGSF抗体, 抗Tslc1抗体
• MDL番号: MFCD07370522
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous solution
• 分子量: antigen 48 kDa
• 化学種の反応性: mouse, rat
• テクニック: immunohistochemistry: suitable using suitable for IHC on rat brain perfused with 4% paraformaldehyde; microarray: suitable; western blot: 0.5-1.0 μg/mL using mouse brain and rat brain extract (S1 fraction)
• UniProtアクセッション番号: Q8R5M8
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: mouse ... Cadm1(54725) ; rat ... Cadm1(363058)

詳細

詳細

Anti-SynCAM is developed in rabbit using a synthetic peptide corresponding to amino acids located at the C-terminus of mouse SynCAM1 conjugated to keyhole limpet hemocyanin (KLH) as immunogen. Synaptic cell adhesion molecule (SynCAM) is a brain-specific protein which has an immunoglobulin domain. It binds to intracellular PDZ-domain protein. SynCAMs have also been called nectin-like molecules (Necls). SynCAM1 is also known as Necl-2/ IGSF4/ RA175/ SgIGSF/ TSLC1. SynCAM contains an N-terminal signal peptide, three extracellular Ig domains, a single transmembrane region and a short C-terminal cytoplasmic domain. It is expressed only in brain and is localized to synapses.

SynCAM proteins consist of a C-terminal cytoplasmic domain, an N-terminal signaling domain and three extracellular immunoglobulin domains.. Anti-SynCAM antibody is specific for SynCAM in mouse and rats. The SynCAM1 immunizing peptide (mouse, amino acids 431-445) specifically inhibits the staining of the SynCAM band in immunoblotting.

免疫原

マウスSynCAM1のC末端に位置するアミノ酸431-445に相当する合成ペプチドのKLH結合体。

This immunogen sequence is identical in human SynCAM1, highly conserved (90%) in the splice variant SynCAM2 and displays limited homology (55%) to SynCAM3.

アプリケーション

• Anti-SynCAM antibody produced in rabbit has been used in:
• immunoprecipitation
• immunoblotting
• immunohistochemistry
• immunostaining
• immunofluorescence

生物化学的／生理学的作用

SynCAM is a neurological cell-adhesion protein that regulates the formation of synapses. Expression of synaptic cell adhesion molecule (SynCAM) in non-neuronal cells induces synaptic formation and affects synaptic function in hippocampal neurons. It functions as a homophilic cell adhesion molecule at the synapse. SynCAM is believed to interact with PDZ-domain synaptic proteins CASK and syntenin via its cytoplasmic tail.

物理的形状

0.01M PBS溶液 (pH 7.4, 15mMアジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)