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Merck

P7899

Sigma-Aldrich

抗ペルオキシダーゼ ウサギ宿主抗体

fractionated antiserum, lyophilized powder

別名:

Rabbit Anti HRP

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About This Item

MDL番号:
UNSPSCコード:
12352203
NACRES:
NA.46

由来生物

rabbit

品質水準

結合体

unconjugated

抗体製品の状態

fractionated antiserum

抗体製品タイプ

primary antibodies

クローン

polyclonal

形状

lyophilized powder

化学種の反応性

horseradish

テクニック

indirect ELISA: 1:100,000-1:200,000

保管温度

2-8°C

ターゲットの翻訳後修飾

unmodified

詳細

Antiserum is determined to be immunospecific for horseradish peroxidase and does not inhibit the enzymatic activity of the peroxidase.
Peroxidase is a hemoprotein.
The fractionation procedure yields primarily the immunoglobulin fraction of antiserum. If necessary, the fractionated antiserum is adsorbed using solid phase techniques. Antiserum is determined to be immunospecific by immunoelectrophoresis (IEP), against purified and crude horseradish peroxidase
The gel is stained with DAB (diaminobenzidine) to ensure that the antibody does not inhibit the enzymatic activity of the peroxidase. Identity and purity of the antibody is established by immunoelectrophoresis. Electrophoresis of the antibody preparation followed by diffusion against anti-rabbit IgG and anti-rabbit whole serum results in single arcs of precipitation in the γ region
Each mL will precipitate a minimum of 0.25 mg of peroxidase at equivalence. May be used to stain axons and synaptic terminals in Drosophila embryo fillets.

免疫原

Purified peroxidase from horseradish

アプリケーション

Anti-Peroxidase antibody produced in rabbit has been used in:
  • immunoblot assays
  • enzyme-linked-immunosorbent assay (ELISA)
  • immunoprecipitation

生物化学的/生理学的作用

Peroxidase helps in catalyzing the oxidation of a number of substrates with the help of hydrogen peroxide.

物理的形状

凍結乾燥品 (0.01M PBS, pH 7.2)。

再構成

脱イオン水で復元してください。

アナリシスノート

特異性は、ペルオキシダ-ゼ粗抽出物を用いた免疫電気泳動で測定しています。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類コード

11 - Combustible Solids

WGK

WGK 3

引火点(°F)

Not applicable

引火点(℃)

Not applicable

個人用保護具 (PPE)

Eyeshields, Gloves, type N95 (US)


試験成績書(COA)

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Rudi Loesel et al.
Journal of neuroscience methods, 155(2), 202-206 (2006-02-17)
Being able to discriminate between neurons and non-neuronal cells such as glia and tracheal cells has been a major problem in insect neuroscience, because glia-specific antisera are available for only a small number of species such as Drosophila melanogaster and
Tyrosine nitration of cytosolic peroxidase is probably triggered as a long distance signaling response in sunflower seedling cotyledons subjected to salt stress
Jain P and Bhatla SC
PLoS ONE, 13(5), e0197132-e0197132 (2018)
Bin Yang et al.
Oncology letters, 15(4), 4662-4668 (2018-03-16)
The present study aimed to assess whether different anesthesia methods (general anesthesia and general anesthesia combined with epidural block) were associated with tumor metastasis during the perioperative period and the possible molecular mechanisms of tumor metastasis. A rat hepatoma tumor
Chilling injury and peroxidase activity changes in ?Fortune? mandarin fruit during low temperature storage
El-HF, et al.
Bulgarian Journal of Plant Physiology, 29(1-2), 44-54 (2003)
Mary Lynn Dear et al.
Development (Cambridge, England), 143(1), 75-87 (2015-11-26)
Synaptogenesis requires orchestrated intercellular communication between synaptic partners, with trans-synaptic signals necessarily traversing the extracellular synaptomatrix separating presynaptic and postsynaptic cells. Extracellular matrix metalloproteinases (Mmps) regulated by secreted tissue inhibitors of metalloproteinases (Timps), cleave secreted and membrane-associated targets to sculpt

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