P6374

p53 ヒト

recombinant, expressed in baculovirus infected Sf21 cells

• UNSPSCコード: 12352202
• NACRES: NA.25

特性

• 由来生物: human
• 品質水準: 200
• リコンビナント: expressed in baculovirus infected Sf21 cells
• アッセイ: ≥90% (SDS-PAGE)
• フォーム: aqueous solution
• テクニック: dot blot: suitable
• UniProtアクセッション番号: P04637
• アプリケーション: genomic analysis
• 輸送温度: dry ice
• 保管温度: −70°C
• 遺伝子情報: human ... TP53(7157)

詳細

詳細

p53 protein is composed of an N-terminal domain with a transactivation domain and proline-rich region, a central core-DNA binding domain, and a C-terminal domain with tetramerization and regulatory domains. The TP53 gene which encodes for the p53 protein is mapped on the human chromosome at 17p13.1.

アプリケーション

p53の翻訳後修飾やタンパク質間相互作用に関する研究に適しています。

p53 human has been used in dot blot.

生物化学的／生理学的作用

In addition, p53 was recently shown to induce differentiation of human embryonic stem cells. The effect is dependent on the DNA binding activity of p53.

p53 gene is highly conserved and expressed in normal tissues. It is the most commonly mutated gene in human cancer and more then 500 gene mutations have been described in various types of malignancies, hematologic as well as solid tumors. Intact p53 function is essential for the maintenance of the non-tumorogenic phenotype of cells. Thus, p53 plays a vital role in suppressing the development of cancer.

p53 protein acts as a transcription factor that participates in regulating positively or negatively the expression of several responsive genes. It plays a role in regulating angiogenesis, cellular senescence, autophagy, cell survival, differentiation, and oxidative stress. Mutations in the TP53 gene lead to Li Fraumeni cancer syndrome.

物性

ヒスチジンで標識されたヒト完全長p53

物理的形状

20 mM Tris-HCl溶液(pH 8.0, 20% グリセロ-ル, 100 mM KCl, 0.2 mM EDTA, 1 mM DTT含有)として販売しております。

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable