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Merck

P3237

Sigma-Aldrich

モノクローナル抗PAK1, リン酸化型(pThr212)抗体 マウス宿主抗体

clone PK-18, purified from hybridoma cell culture

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About This Item

MDL番号:
UNSPSCコード:
12352203
NACRES:
NA.44

由来生物

mouse

結合体

unconjugated

抗体製品の状態

purified from hybridoma cell culture

抗体製品タイプ

primary antibodies

クローン

PK-18, monoclonal

形状

buffered aqueous solution

分子量

antigen 68 kDa

化学種の反応性

human, mouse, rat

濃度

~2 mg/mL

テクニック

immunocytochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.2-0.4 μg/mL using a whole extract of transfected and activated COS-7 (monkey kidney) cells expressing phosphorylated PAK1

アイソタイプ

IgG1

UniProtアクセッション番号

輸送温度

dry ice

保管温度

−20°C

ターゲットの翻訳後修飾

phosphorylation (pThr212)

遺伝子情報

human ... PAK1(5058)
mouse ... Pak1(18479)
rat ... Pak1(29431)

詳細

Monoclonal Anti-Phospho-PAK1 (pThr212) (mouse IgG1 isotype) is derived from the PK-18 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino acids of human PAK1,1 conjugated to keyhole limpet hemocyanin (KLH). PAK1 (p21-activated kinase 1) is homologous to Sacharomyces cerevisiae kinase Ste20. PAK1 (68 kDa),with a C-terminal serine/threonine kinase domain and a N-terminal CRIB (Cdc42/Rac interactive binding) domain. Thr212 localized in flexible region is of PAK1 domain.
p21-activated kinases are a family of highly conserved serine/threonine protein kinases that are activated by small GTPases of Cdc42 and Rac families. There are 2 groups of PAKs in mammals, group A and B. The group A members are three isoforms, PAK1, PAK2, and PAK3 (also known as α-PAK, γ-PAK, and β-PAK, respectively). PAKs are actively involved in regulation of MAPK pathways by phosphorylation of Raf-1 and MEK-1. They also regulate cytoskeletal reorganization by interaction with GTPases. Autophosphorylation of threonine 423 (threonine 402 for PAK2 and threonine 421 for PAK3), catalyzed by Cdc42, is required for activation of PAK. In the neurons, the reorganization of cytoskeleton during the neurite outgrowth is modulated by phosphorylation of PAK1 at Thr212 that regulates a specific signalling pathway
Monoclonal Anti-Phospho-PAK1 (pThr212) reacts specifically with human PAK1 phosphorylated at Thr212 (68 kDa), and does not detect the unphosphorylated epitope.

免疫原

ヒトPAK1のアミノ酸 203-217(pThr212)に相当する合成ペプチド。

アプリケーション

Anti-phospho-PAK1 (pThr212) antibody, Mouse monoclonal has been used in:
  • enzyme linked immunosorbent assay (ELISA)
  • immunoblotting
  • immunohistochemistry


Anti-phospho-PAK1 (pThr212) may be used for immunoblotting at a working concentration of 0.2-0.4 μg/mL. The antibody is also suitable for indirect ELISA, immunocytochemistry and protein microarray.

生物化学的/生理学的作用

p21-activated kinase 1 (PAK1) also regulates cytoskeletal dynamics by decreasing MLCK (myosin light chain kinase) activity and MLC phosphorylation. PAK1 is reported to be a crucial signaling molecule, involved in the transcription factor nuclear factor κB (NFκB) activation by multiple stimuli. PAK1 regulates cell morphology and polarity in eukaryotic cells and activates downstream targets like JNK (c-Jun N-terminal protein kinase) and p38 mitogen-activated protein kinase (MAPK).. The phosphorylation of actin by PAK1 at thr212 helps in regulating growth cone dynamics.

物理的形状

0.01M PBS溶液 (pH 7.4, 15mMアジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類コード

10 - Combustible liquids

WGK

WGK 3

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

P3237-VAR:
P3237-.2ML:
P3237-BULK:


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Activating mutations in PAK1, encoding p21-activated kinase 1, cause a neurodevelopmental disorder
Harms FL, et al.
American Journal of Human Genetics, 103(4), 579-591 (2018)
Activation of p21-activated kinase 1-nuclear factor kappaB signaling by Kaposi?s sarcoma-associated herpes virus G protein-coupled receptor during cellular transformation
Dadke D, et al.
Cancer Research, 63(24), 8837-8847 (2003)
Susanne Hipp et al.
Journal of cellular and molecular medicine, 13(9B), 3858-3867 (2009-07-17)
Reduced E-cadherin expression is associated with tumour progression of many carcinomas, including endometrial cancers. The transcription factor Snail is known as one of the most prominent transcriptional E-cadherin repressors; its regulation in cancer tissues, however, still remains unclear. Here, we
ERK activation of p21 activated kinase-1 (Pak1) is critical for medulloblastoma cell migration
Yuan L, et al.
Clinical & Experimental Metastasis, 27(7), 481-481 (2010)
Phosphorylation of Pak1 by the p35/Cdk5 kinase affects neuronal morphology
Rashid T, et al.
Test, 276(52), 49043-49052 (2001)

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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