P302-05

Porcine Pulmonary Artery Endothelial Cells: PPAEC (Cryovial)

• UNSPSCコード: 41106514
• NACRES: NA.81

特性

• 由来生物: Porcine pulmonary artery (Normal healthy)
• 品質水準: 100
• フォーム: solid
• 包装: pkg of 500,000 cells
• メーカー／製品名: Cell Applications, Inc
• 成長モード: Adherent
• 核型: 2n = 38
• 形態: Endothelial
• テクニック: cell culture | mammalian: suitable
• 関連疾患: diabetes; cardiovascular diseases
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

詳細

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

PPAEC have been used in a pioneer study of endothelial cell motility, where it was determined that asymmetric dimethylarginine (ADMA), an inhibitor of nitric oxide production and a cardiovascular risk factor, decreased PKG-mediated phosphorylation of VASP, leading to downregulation of Rac1 and induction of stress fibers and focal adhesions via RhoA/ROCK dependent mechanism, leading to decreased cell motility, and increase in endothelial permeability, thus linking vascular repair and function with NO production (Wojciak-Stothard, 2007, 2009). PPAEC were also used to assess endothelial adhesion to decellularized porcine arteries in order to develop grafts for human vascular repair (Xiong, 2013).

細胞株の由来

Artery

アプリケーション

cardiovascular function, screening of drug candidates, angiogenesis, vasodilator function, transport of deoxygenated bloood from heart to lungs

構成

Porcine Endothelial Cell Growth Medium that contains 10% FBS and 10% DMSO

調製ノート

• 1st passage, >500,000 cells in Porcine Endothelial Cell Growth Medium that contains 10% FBS and 10% DMSO
• Can be cultured at least 16 doublings

継代と培養方法

Please refer to the PPAEC Culture Protocol.

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable