P0073

Anti-PERK (N-terminal) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• 別名: Anti-E2AK3, Anti-EIF2AK3, Anti-HsPEK, Anti-PEK, Anti-PRKR-like endoplasmic reticulum kinase, Anti-Pancreatic eIF2α kinase, Anti-WRS
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 結合体: unconjugated
• 抗体製品の状態: IgG fraction of antiserum
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous solution
• 交差性: mouse, rat
• テクニック: western blot: 1:500-1:1,000 using whole cell lysates of HEK-293T expressing PERK
• UniProtアクセッション番号: Q9Z1Z1
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: mouse ... Eif2ak3(13666) ; rat ... Eif2ak3(29702)

詳細

詳細

Protein kinase RNA-like endoplasmic reticulum kinase (PERK) is one of the three mammalian UPR (unfolded protein response) transducers; the other two being activating transcription factor 6 (ATF6), and inositol-requiring enzyme 1 (IRE1). It is a highly conserved eIF2a (eukaryotic initiation factor 2 α) kinase.

特異性

Anti-PERK (N-terminal) specifically recognizes mouse and rat PERK.

免疫原

synthetic peptide corresponding to amino acids 124-139 of mouse PERK, conjugated to KLH. This sequence is identical in rat and mouse.

アプリケーション

Anti-PERK (N-terminal) antibody produced in rabbit may be used in immunoblotting.

生物化学的／生理学的作用

Protein kinase RNA-like endoplasmic reticulum kinase (PERK) is an ER stress sensor which phosphorylates eukaryotic initiation factor 2 α (eIF2α), thereby reducing the translation of most messenger RNAs (mRNAs). This results in reduction of protein load in the ER lumen. In S63del-Charcot-Marie-Tooth 1B neuropathy mice model, the suppression of PERK results in improved myelination. In mice models of frontotemporal dementia, the suppression of translation in a PERK-eIF2α-dependent manner plays a key role in neuronal loss. Administration of GSK2606414, a PERK inhibitor, results in suppression of tau-mediated neurodegeneration. PERK confers protection to heart against pressure overload-induced congestive heart failure. Mutations in this gene are linked with Wolcott-Rallison syndrome (WRS) in humans, which is characterized by permanent neonatal diabetes, exocrine-deficient pancreas, growth retardation, and osteopenia.

ターゲットの説明

PERK (also known as eIF2AK3, PEK and WRS) is a transmembrane kinase that is highly expressed in thepancreas. It resides in the ER and couples stress signals initiated by protein malfolding in the ER lumen to eIF2α phosphorylation and reduces protei

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

保管および安定性

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)