N5037

Anti-Neurabin II (C-terminal) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• 別名: Anti-Neural-tissue Specific F-actin Binding Protein II, Anti-PP1bp134, Anti-Protein Phosphatase 1 Regulatory Subunit 9B, Anti-Spinophilin
• MDL番号: MFCD02686629
• UNSPSCコード: 12352203
• NACRES: NA.43

特性

• 由来生物: rabbit
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous solution
• 分子量: antigen 140 kDa
• 交差性: rat, mouse, canine
• テクニック: immunoprecipitation (IP): 10-20 μg using rat brain extract (S1 fraction); indirect immunofluorescence: 10-20 using MDCK cells.; microarray: suitable; western blot: 1-2 μg/mL using mouse and rat brain extracts (S1 fraction)
• UniProtアクセッション番号: O35274
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... PPP1R9B(84687) ; mouse ... Ppp1r9b(217124) ; rat ... Ppp1r9b(84686)

詳細

詳細

Neurabin II belongs to a family of F-actin-binding proteins, highly enriched in dendritic spines and involved in neurite formation. It consists of an F-actin binding domain at the N-terminus, a single PDZ-interacting-domain and a C-terminal coiled-coil domain.

免疫原

synthetic peptide corresponding to amino acids 800-817 located at the C-terminal region of rat neurabin II. The sequence is identical in human and mouse neurabin II and not found in neurabin I.

アプリケーション

Anti-Neurabin II (C-terminal) antibody has been used in

• immunoblotting
• immunoprecipitation
• immunocytochemistry

生物化学的／生理学的作用

Neurabins appear to function as bridging proteins by targeting other proteins to the synapse or by linking plasma membrane proteins to the actin cytoskeleton. Neurabin II binds to several proteins including F-actin, protein phosphatase 1 (PP1), at least two subfamilies of G-protein coupled receptors (GPCRs), the α2 adrenergic receptor (α2AR) subtypes and the D2 dopamine receptor. Neurabin II blocks arrestin function in vitro and in vivo at GPCRs and has been also shown to modulate, in vitro and in vivo, both glutaminergic synaptic transmission and the formation and function of dendritic spines. Neurabin II and neurabin I target PP1 subunits that are highly concentrated in dendritic spines and post-synaptic densities. Phosphorylation of neurabin II modulates its interaction with actin filaments, via the anchoring of the neurabin II-PP1 complex within dendritic spines, thereby contributing to the efficacy and plasticity of synaptic transmission.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable