MAK529

LDH Cytotoxicity Assay Kit

sufficient for 100 colorimetric tests

• 別名: LDH-Cytotoxicity Test Kit II
• UNSPSCコード: 12161503
• NACRES: NA.84

特性

• 品質水準: 100
• 検出方法: colorimetric
• 関連疾患: hematological disorder; cancer
• 輸送温度: wet ice
• 保管温度: −20°C

詳細

詳細

Lactate dehydrogenase (LDH) is an oxidoreductase which catalyzes the interconversion of lactate and pyruvate. Cytotoxic compounds often compromise cell membrane integrity by inducing apoptosis or necrosis. LDH is a stable cytosolic enzyme, that upon membrane damage, is released into the cellular environment. Therefore, LDH is often measured to evaluate the presence of tissue or cell damage.

特徴および利点

• Compatible with high-throughput handling systems.

適合性

The kit is suitable for the rapid quantitative determination of cytotoxicity based on lactate dehydrogenase released into cell culture medium, and for the evaluation of toxic compounds, toxins, detergents, environmental pollutants and physical treatment on cell lysis.

原理

The LDH Cytotoxicity Assay Kit is a simple and robust method to assess cytotoxic effects on cells by measuring the activity of LDH in cell culture supernatant. The assay is based on the reduction of a tetrazolium salt to a formazan dye.

安全性情報

• ピクトグラム: GHS05,GHS07
• シグナルワード: Danger
• 危険有害性情報: H302 + H332,H315,H318
• 注意書き: P261 - P280 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
• 危険有害性の分類: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2
• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3