MAK512

Malate Dehydrogenase Assay Kit

sufficient for 100 colorimetric tests

• UNSPSCコード: 12352204
• NACRES: NA.84

特性

• 品質水準: 100
• 検出方法: colorimetric
• 関連疾患: Alzheimer′s disease; neurological disorders; neurodegenerative disorders
• 輸送温度: wet ice
• 保管温度: −20°C

詳細

詳細

Malate dehydrogenase (MDH) is an enzyme which reversibly catalyzes the oxidation of L-malate to oxaloacetate in the presence of NAD. There are 2 isoforms in eukaryotic cells: MDH1 and MDH2. MDH1 is found in the cytoplasm and plays a key part in the malateaspartate shuttle for transporting malate into the mitochondria. MDH2 is a mitochondrial enzyme which participates in the TCA cycle that reversibly converts L-malate into oxaloacetate. Higher MDH activities are found in some neurodegenerative diseases such as Alzheimer′s disease.

特徴および利点

• Linear detection range: 0.5 to 65 U/L
• Compatible with high-throughput handling systems.

適合性

The kit is suitable for malate dehydrogenase activity determination in biological samples (e.g. plasma, serum, erythrocytes, tissue and culture media.)

原理

The Malate Dehydrogenase Assay Kit is a non-radioactive, colorimetric MDH assay based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.

安全性情報

• ピクトグラム: GHS02,GHS05,GHS07
• シグナルワード: Danger
• 危険有害性情報: H225,H290,H315,H319,H336
• 注意書き: P210 - P233 - P234 - P240 - P303 + P361 + P353 - P305 + P351 + P338
• 危険有害性の分類: Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
• ターゲットの組織: Central nervous system
• 保管分類コード: 3 - Flammable liquids
• WGK: WGK 3