MAK505

Ascorbic Acid Assay Kit

sufficient for 100 colorimetric or fluorometric tests

• UNSPSCコード: 12161503
• NACRES: NA.84

特性

• 品質水準: 100
• アプリケーション: food and beverages; pharmaceutical
• 検出方法: colorimetric; fluorometric
• 輸送温度: wet ice
• 保管温度: −20°C

詳細

詳細

Ascorbic acid (the L-enantiomer commonly known as vitamin C) is an important antioxidant found in living organisms and applied as additives in food and other industrial processes. By reacting with reactive oxygen species, it protects the cell from oxidative damage.

特徴および利点

• Linear detection range: 6 to 1000 µM for the colorimetric assay and 1 to 100 µM for the fluorometric assay.

適合性

The kit is suitable for ascorbic acid determination in biological samples such as serum, plasma, urine, saliva, milk, tissue, and cell culture, as well as for studying the effects of drugs on ascorbic acid metabolism.

原理

The Ascorbic Acid Assay Kit provides a simple, direct and high-throughput assay for measuring ascorbic acid. In this assay, ascorbic acid is oxidized by ascorbate oxidase resulting in the production of H2O2 which reacts with a specific dye to form a pink colored product. The color intensity at 570 nm or fluorescence intensity at λEx = 530 nm/λEm = 585 nm is directly proportional to the ascorbic acid concentration in the sample.

安全性情報

• ピクトグラム: GHS07
• シグナルワード: Warning
• 危険有害性情報: H319
• 注意書き: P264 - P280 - P305 + P351 + P338 - P337 + P313
• 危険有害性の分類: Eye Irrit. 2
• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2