MAK336

D-Lactate Assay Kit

sufficient for 100 colorimetric tests

• 別名: D-Lactate Detection Kit
• UNSPSCコード: 12161503
• NACRES: NA.84

特性

• 使用法: sufficient for 100 colorimetric tests
• 入力: cell medium; serum; plasma
• 検出方法: colorimetric
• 関連疾患: neurological disorders; gastrointestinal diseases
• 保管温度: −20°C

詳細

詳細

Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. D-lactate is produced in only minor quantities in animals and measuring for D-lactate in animal samples is a means to determine the presence of bacterial infection. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable.

アプリケーション

The D-Lactate Assay Kit may be used for:

• Gastrointestinal Disease Research
• Neurological Disease Research

特徴および利点

Enhanced Detection Range: Detect D-Lactate levels accurately across a wide range, from 0.05 mM to 2 mM, using only 20 μL of sample in a 96-well plate setup, ensuring comprehensive analysis with minimal sample requirement.

Simplified Process: Experience a streamlined process with the addition of only a single working reagent and a 20 minute room temperature reaction, reducing complexity and saving valuable time and effort.

Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

適合性

Suitable for D-lactate in serum, plasma, and cell media samples.

原理

The D-Lactate Assay Kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) Reagent. The intensity of the product color, measured at 565 nm, is proportionate to the lactate concentration in the sample. Detection limit of 0.05 mM and linearity up to 2 mM D-lactate in 96-well plate assay. For cell culture samples containing phenol red: detection limit of 0.1 mM and linearity up to 1 mM D-lactate in 96-well plate assay.

その他情報

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安全性情報

• 保管分類コード: 10 - Combustible liquids