M8070

モノクローナル抗リン酸化MDMX (pTyr⁵⁵) 抗体 マウス宿主抗体

~2 mg/mL, clone PH-MDMX-55, purified immunoglobulin, buffered aqueous solution

• 別名: 抗HDM4抗体, 抗HDMX抗体, 抗MDM4抗体, 抗MGC132766抗体, 抗MRP1抗体
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 結合体: unconjugated
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: PH-MDMX-55, monoclonal
• 形状: buffered aqueous solution
• 分子量: antigen ~80 kDa
• 化学種の反応性: human
• 包装: antibody small pack of 25 μL
• 濃度: ~2 mg/mL
• テクニック: indirect ELISA: suitable; western blot: 4-8 μg/mL using total cell extract of HEK-293T cells co-transfected with human MDMX and a specific kinase
• アイソタイプ: IgG2a
• UniProtアクセッション番号: O15151
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: phosphorylation (pTyr55)
• 遺伝子情報: human ... MDM4(4194)

詳細

詳細

Monoclonal Anti-phospho-MDMX (pTyr55) (mouse IgG2a isotype) is derived from the hybridoma PH-MDMX-55 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Mouse Double Minute 4 (MDM4) comprises the N-terminal p53 binding domain, a zinc finger, and an acidic region, followed by a really interesting new gene (RING) domain at C-terminus. MDM4 gene is mapped to human chromosome 1q32.1. The RING finger harbors a putative nuclear localization signal.

特異性

Monoclonal Anti-phospho-MDMX (pTyr55) recognizes human phosphorylated MDMX (pTyr55) (approx. 80 kDa).

免疫原

ヒトMDMXのアミノ酸50~64 (Tyr⁵⁵リン酸化) に相当する合成リン酸化ペプチド。

アプリケーション

Monoclonal Anti-phospho-MDMX (pTyr55) antibody produced in mouse may be used in:

• immunoblotting
• immunoprecipitation
• immunocytochemistry
• enzyme-linked immunosorbent assay (ELISA)

生物化学的／生理学的作用

Mouse Double Minute 4 (MDM4 or HDMX or MDMX) undergoes phosphorylation. It is the major inhibitor of p53 transcriptional activity. Despite their significant structural similarity, mouse double minute 2 (MDM2) and MDMX play distinct roles in p53 regulation. The critical and non-redundant role of MDMX and MDM2 in the regulation of p53 in vivo is strikingly demonstrated by the lethality of the mdmx or mdm2 null embryos, which can be fully rescued by the elimination of p53. MDM2 or MDMX are frequently overexpressed in human cancers, leading to the suppression of p53. Studies on MDMX in response to stress conditions, involving post-translational modifications and protein-protein interactions are reported.

物理的形状

0.01 M PBS溶液 (pH 7.4, 15 mM アジ化ナトリウム含有)。

保管および安定性

For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discard if not used within 12 hours.

免責事項

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)