M6693

モノクローナル抗MAT-タグ^®抗体 マウス宿主抗体

purified immunoglobulin, ~2 mg/mL, clone MAT1-87

• MDL番号: MFCD06798711
• UNSPSCコード: 12352203
• NACRES: NA.56
• クローン: MAT1-87, monoclonal
• application: IP; WB
• テクニック: immunoprecipitation (IP): 5-10 μg; western blot: 1-2 μg/mL
• citations: 5

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: MAT1-87, monoclonal
• 濃度: ~2 mg/mL
• テクニック: immunoprecipitation (IP): 5-10 μg; western blot: 1-2 μg/mL
• アイソタイプ: IgG2a
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

Monoclonal Anti-MAT (mouse IgG2a isotype) is derived from the MAT1-87 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic MAT peptide HNHRHKHGGGC conjugated to KLH.

Monoclonal Anti-Mat-Tag^® antibody was used in one-step purification of histidine-tagged fusion proteins under high pressure and high flow conditions. It is used in the characterization of the molecular architecture of human caveolin-3 and interaction with the skeletal muscle ryanodine receptor.

特異性

Monoclonal Anti-MAT recognizes the seven amino acid MAT epitope sequence within either N or C terminal MAT-tagged fusion proteins.

免疫原

合成MATペプチドHNHRHKHGGGC。

アプリケーション

Monoclonal Anti-MAT-Tag^® antibody produced in mouse has been used in:

• enzyme linked immunosorbent assay (ELISA)
• immunoblotting
• immunoprecipitation
• immunocytochemistry

MATタグは、7アミノ酸からなるペプチドタグで、ニッケルやコバルトなど遷移金属と結合します。このタグにはHNHRHKHの配列が含まれ、高感度HIS-Select^®ニッケルアフィニティ-ゲルなどの固定化金属アフィニティ-クロマトグラフィ-(IMAC)を用いたMAT融合タンパク質の精製が可能です。MATタグ配列は、原核および真核細胞発現ベクタ-のいずれにも組み込めます。

物理的形状

Supplied as a solution in 0.01M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative.

保管および安定性

For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

法的情報

HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany

MAT-Tag is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable