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由来生物
rabbit
結合体
unconjugated
抗体製品の状態
affinity isolated antibody
抗体製品タイプ
primary antibodies
クローン
polyclonal
形状
buffered aqueous solution
分子量
antigen ~73 kDa
化学種の反応性
human, rat, mouse
テクニック
indirect immunofluorescence: 5-10 μg/mL using human HepG2 cells or rat NRK cells
western blot (chemiluminescent): 0.5-1 μg/mL using whole extract of mouse NIH3T3 cells
UniProtアクセッション番号
輸送温度
dry ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... MAN1A1(4121)
mouse ... Man1a(17155)
rat ... Man1a1(294410)
詳細
α1,2-Mannosidase IA is a type II transmembrane Golgi-resident enzyme that belongs to class I α1,2-Mannosidases (glycosylhydrolase family 47). The highly conserved Class I α1,2-Mannosidases classified into three subgroups according to their enzymatic activities. α1,2-Mannosidase IA is predominantly detected in the juxtanuclear Golgi region. The three subgroups differ in their expression pattern, subcellular localization, and substrate specificity.
免疫原
synthetic peptide corresponding to amino acids 138-154 of human α1,2-mannosidase IA with N-terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in mouse.
アプリケーション
Anti-α1,2-Mannosidase IA antibody produced in rabbit has been used in:
- immunohistochemistry
- immunoblotting
- immunofluorescence
生物化学的／生理学的作用
α1,2-Mannosidase IA belongs to the first subgroup out of the three subgroups of class I α1,2-Mannosidases. The first subgroup consists of yeast and human α 1,2-mannosidases of the endoplasmic reticulum that convert Man9GlcNAc2 to Man8GlcNAc2 isomer B. The second subgroup comprising of mammalian Golgi α1,2-Mannosidases IA, IB, and IC cleave Man9GlcNAc2 to Man5GlcNAc2 via Man8GlcNAc2 isomer A and C. The third subgroup of yeast and mammalian proteins do not show any activity on Man9GlcNAc2. Proteins from subgroup 1 and 3 have been implicated in ER quality control and in proteasomal degradation of misfolded glycoproteins. The Golgi mannosidases from the second subgroup may play a role in the ERAD (endoplasmic reticulum-associated degradation) of defective glycoproteins. The three subgroups have been found to pariticipate in the maturation of Asn-linked glycoproteins in the endoplasmic reticulum (ER) and Golgi complex.
物理的形状
0.01M PBS溶液 (1%BSA, 15mMアジ化ナトリウム含有)。
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
IXO11669:
M3694-200UL:
M3694-BULK:
M3694-VAR:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Identification of cholinergic chemosensory cells in mouse tracheal and laryngeal glandular ducts
Krasteva-Christ G, et al.
International Immunopharmacology, 29(1), 158-165 (2015)
O-GlcNAcylation mediates metastasis of cholangiocarcinoma through FOXO3 and MAN1A1
Phoomak C, et al.
Oncogene, 37(42), 5648-5648 (2018)
A Herscovics
Biochimie, 83(8), 757-762 (2001-09-01)
Class I alpha 1,2-mannosidases (glycosylhydrolase family 47) are conserved through eukaryotic evolution. This protein family comprises three subgroups distinguished by their enzymatic properties. The first subgroup includes yeast (Saccharomyces cerevisiae) and human alpha 1,2-mannosidases of the endoplasmic reticulum that primarily
Yuri D Lobsanov et al.
The Journal of biological chemistry, 277(7), 5620-5630 (2001-11-21)
Class I alpha1,2-mannosidases (glycosylhydrolase family 47) are key enzymes in the maturation of N-glycans. This protein family includes two distinct enzymatically active subgroups. Subgroup 1 includes the yeast and human endoplasmic reticulum (ER) alpha1,2-mannosidases that primarily trim Man(9)GlcNAc(2) to Man(8)GlcNAc(2)
Jie Li et al.
Molecular biology of the cell, 30(4), 478-490 (2018-12-20)
In mammalian cells, the Golgi reassembly stacking protein of 65 kDa (GRASP65) has been implicated in both Golgi stacking and ribbon linking by forming trans-oligomers. To better understand its function and regulation, we used biochemical methods to identify the DnaJ
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