I5283

抗INCENP抗体 ウサギ宿主抗体

IgG fraction of antiserum, buffered aqueous solution

• 別名: 抗内部セントロメアタンパク質抗体
• MDL番号: MFCD05665235
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 結合体: unconjugated
• 抗体製品の状態: IgG fraction of antiserum
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: buffered aqueous solution
• 分子量: antigen ~106 kDa
• 化学種の反応性: human, mouse, rat
• テクニック: indirect immunofluorescence: 1:100 using HeLa cells; microarray: suitable; western blot (chemiluminescent): 1:2,000 using whole extract of mouse NIH3T3 and rat PC12 cells; western blot (chemiluminescent): 1:5,000 using nuclear extract of human HeLa cells
• UniProtアクセッション番号: Q9NQS7
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... INCENP(3619) ; mouse ... Incenp(16319) ; rat ... Incenp(293733)

詳細

詳細

The gene INCENP (inner centromere protein) encodes an inner centromere protein that forms a component of the chromosomal passenger complex (CPC). It is found to be localized to inner centromeres during mitosis. The CPC also contains Aurora B, survivin, and Borealin.

免疫原

ヒトINCENPのアミノ酸配列884-901に相当する合成ペプチド(N末端システイン付加)のKLH結合体。相当する配列はニワトリおよびアフリカツメガエル(Xenopus laevis)と1アミノ酸、マウスと2アミノ酸異なっています。

アプリケーション

Anti-INCENP antibody produced in rabbit has been used in western blotting and immunofluorescence.

生物化学的／生理学的作用

Studies in knockout mice established INCENP (inner centromere protein) as essential for mouse development and viability. Loss of INCENP results in chromosome segregation defects and failures in cytokinesis.

The inner centromere protein encoded by the INCENP gene is phosphorylated by Cdk1 (cyclin dependent kinase 1) in mitosis. It is found to be associated with Plk1 (Polo-like kinase) in a polo-box–dependent manner. Plk1 is a serine/threonine kinase that functions in cell cycle, especially in mitosis. INCEP participates in the localization of Bub1 (Budding uninhibited by benzimidazole 1), a serine/threonine kinase involved in spindle checkpoint, to kinetochore. This, in turn, promotes the recruitment of Plk1 to kinetochores. Phosphorylated INCENP stimulates the activity of Aurora B kinase at centromeres and the central spindle that is important for chromosome segregation and cytokinesis, respectively.

物理的形状

0.01M PBS溶液 (pH 7.4, 15mMアジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)