HPA018433

抗CBR1抗体 ウサギ宿主抗体

affinity isolated antibody, buffered aqueous glycerol solution

• 別名: Anti-15-Hydroxyprostaglandin dehydrogenase [NADP+], Anti-Carbonyl reductase [NADPH] 1, Anti-NADPH-dependent carbonyl reductase 1, Anti-Prostaglandin 9-ketoreductase, Anti-Prostaglandin-E(2) 9-reductase
• UNSPSCコード: 12352203
• Human Protein Atlas Number: HPA018433
• NACRES: NA.43

特性

• 由来生物: rabbit
• 品質水準: 100
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous glycerol solution
• 化学種の反応性: human
• テクニック: immunoblotting: 0.04-0.4 μg/mL; immunohistochemistry: 1:50-1:200
• 免疫原配列: TPFHIQAEVTMKTNFFGTRDVCTELLPLIKPQGRVVNVSSIMSVRALKSCSPELQQKFRSETITEEELVGLMNKFVE
• UniProtアクセッション番号: P16152
• 輸送温度: wet ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... CBR1(873)

詳細

詳細

The gene CBR1 (carbonyl reductase [NADPH] 1) is mapped to human chromosome 21q22.13. CBR1 is a ubiquitously-expressed protein. The protein localizes in the cytoplasm and is monomeric in nature. CBR1 is abundantly present in amnion epithelial, fibroblast cells and chorionic trophoblast cells.

免疫原

カルボニルレダクターゼ[NADPH]1のPrEST(protein epitope signature tag)抗原リコンビナントタンパク質。

アプリケーション

Prestige抗体^®は、Human Proteome Resource(HPR)プロジェクト(www.proteinatlas.org)によって開発・実証されたAtlas抗体です。抗体はすべて、数百の正常組織・疾病組織に対する免疫組織染色試験を行っています。これらの染色画像はHuman Protein Atlas(HPA)サイトで[Image Gallery]リンクをクリックするとご覧いただけます。さらに、ほとんどのPrestige抗体はプロテインアレイおよびウェスタンブロッティングの試験を行っています。試験のプロトコールおよびPrestige抗体、HPAに関する情報はsigma.com/prestigeをご覧ください。

生物化学的／生理学的作用

Carbonyl reductase [NADPH] 1 (CBR1) is an NADPH-dependent dehydrogenase/reductase and is responsible for the in vivo reduction of quinones, prostaglandins and other carbonyl-containing compounds including xenobiotics. In addition nitrogen-containing GSH adduct S-nitrosoglutathione (GSNO) is an efficient substrate of CBR1. CBR1 activity can be inhibited by various flavonoids including quercetin, rutin and semi-synthetic flavonoid monoHER (7-monohydroxyethylrutoside). Curcumin, a major component of the plant Curcuma longa, is a potent tight-binding inhibitor of CBR1. Absence of CBR1 promotes ovarian cancer growth and proliferation. CBR1 is responsible for conversion of cytotoxic daunorubicin to cardiotoxic daunorubicinol, thus reducing cytotoxicity of daunorubicin in primary acute myeloid leukemia cells. Glucocorticoid receptor binding to the CBR1 promoter stimulates CBR1 production in in human amnion fibroblasts. Presence of CBR1 converts prostaglandin E2 to prostaglandin F2α, which is involved in induction of human labor. NRF2 (Nuclear factor erythroid 2-related factor 2) is a transcriptional regulator of CBR1.

特徴および利点

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

関連事項

Corresponding Antigen APREST73872

物理的形状

PBS溶液(pH 7.2, 40%グリセロールおよび0.02%アジ化ナトリウム含有)。

法的情報

Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)