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Merck

HPA014055

Sigma-Aldrich

Anti-IQGAP1 antibody produced in rabbit

enhanced validation

Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

別名:

Anti-Ras GTPase-activating-like protein IQGAP1, Anti-p195

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About This Item

MDL番号:
UNSPSCコード:
12352203
Human Protein Atlas Number:
NACRES:
NA.41

由来生物

rabbit

結合体

unconjugated

抗体製品の状態

affinity isolated antibody

抗体製品タイプ

primary antibodies

クローン

polyclonal

製品種目

Prestige Antibodies® Powered by Atlas Antibodies

形状

buffered aqueous glycerol solution

化学種の反応性

human, mouse, rat

強化検証

orthogonal RNAseq
Learn more about Antibody Enhanced Validation

テクニック

immunoblotting: 0.04-0.4 μg/mL
immunohistochemistry: 1:200-1:500

免疫原配列

NRALESGDVNTVWKQLSSSVTGLTNIEEENCQRYLDELMKLKAQAHAENNEFITWNDIQACVDHVNLVVQEEHERILAIGLINEALDEGDAQKTLQALQIPAAKLEGVLAEVAQHYQDTLIRAKREKAQEIQDESAVLWLDEIQGG

UniProtアクセッション番号

輸送温度

wet ice

保管温度

−20°C

ターゲットの翻訳後修飾

unmodified

遺伝子情報

human ... IQGAP1(8826)

詳細

The gene IQGAP1 (IQ motif containing GTPase activating protein 1) encodes a calmodulin-binding protein that belongs to the IQGAP family and is ubiquitously expressed. The encoded protein contains four IQ domains and a region with sequence similarity to the Ras GTPase-activating proteins. The gene is mapped to human chromosome 15q26.1.

免疫原

Ras GTPアーゼ活性化様タンパク質IQGAP1のPrEST(protein epitope signature tag)抗原リコンビナントタンパク質。

アプリケーション

Prestige抗体®は、Human Proteome Resource(HPR)プロジェクト(www.proteinatlas.org)によって開発・実証されたAtlas抗体です。抗体はすべて、数百の正常組織・疾病組織に対する免疫組織染色試験を行っています。これらの染色画像はHuman Protein Atlas(HPA)サイトで[Image Gallery]リンクをクリックするとご覧いただけます。さらに、ほとんどのPrestige抗体はプロテインアレイおよびウェスタンブロッティングの試験を行っています。試験のプロトコールおよびPrestige抗体、HPAに関する情報はsigma.com/prestigeをご覧ください。

生物化学的/生理学的作用

The gene IQGAP1 (IQ motif containing GTPase activating protein 1) encodes a key effector of Rac1 and Cdc42, Rho-family of small GTPases that regulate E-cadherin–mediated cell-cell adhesion. It functions in the modulation of actin cytoskeleton along with Rac1 and Cdc42. The encoded protein modulates cell–cell adhesion through E-cadherin and β-catenin. It participates in the regulation of mitogen activated protein kinase pathway that is involved in cell proliferation and differentiation. IQGAP1 is a scaffold protein that bridges the proteins involved in signaling cascades and participates in several cellular processes. It binds to and stabilizes microtubules via CLIP-170, a microtubule-binding protein, near the cell cortex. This results in the formation and maintenance of polarized cell morphology and directional cell migration.

特徴および利点

Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:
  • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
  • Protein array of 364 human recombinant protein fragments.

関連事項

Corresponding Antigen APREST72663

物理的形状

PBS溶液(pH 7.2, 40%グリセロールおよび0.02%アジ化ナトリウム含有)。

法的情報

Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類コード

10 - Combustible liquids

WGK

WGK 1

引火点(°F)

Not applicable

引火点(℃)

Not applicable

個人用保護具 (PPE)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

HPA014055-100UL:


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Matthew D Brown et al.
Trends in cell biology, 16(5), 242-249 (2006-04-06)
IQGAP1 was identified in 1994 as a widely expressed IQ domain-containing protein with a region containing sequence similarity to the Ras GTPase-activating proteins. IQGAP1 has roles in many different aspects of cell physiology and interacts with numerous proteins. It modulates
Jun Noritake et al.
Journal of cell science, 118(Pt 10), 2085-2092 (2005-05-14)
The dynamic rearrangement of cell-cell adhesion is one of the major physiological events in tissue development and tumor metastasis. Polarized cell migration, another key event, is a tightly regulated process that occurs during tissue development, chemotaxis and wound healing. Rho-family
M A Pujana et al.
Genome research, 11(1), 98-111 (2001-01-13)
Several cytogenetic alterations affect the distal part of the long arm of human chromosome 15, including recurrent rearrangements between 12p13 and 15q25, which cause congenital fibrosarcoma (CFS). We present here the construction of a BAC/PAC contig map that spans 2
A M Bashour et al.
The Journal of cell biology, 137(7), 1555-1566 (1997-06-30)
Activated forms of the GTPases, Rac and Cdc42, are known to stimulate formation of microfilament-rich lamellipodia and filopodia, respectively, but the underlying mechanisms have remained obscure. We now report the purification and characterization of a protein, IQGAP1, which is likely
Xianchen Huang et al.
International journal of clinical and experimental pathology, 7(10), 6475-6485 (2014-11-18)
Recently, the architectural remodeling of venous vessel wall ranks as the basis of varicose veins development based on the phenotypic state of vascular smooth muscle cells (VSMCs). In this study, we firstly demonstrated an obvious up-regulation of IQ-domain GTPase-activating protein

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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