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ligand
butyl
包装
pack of 1 column
メーカー／製品名
Cytiva 28-9924-73
ベッドサイズ
7.7 mm × 100 mm
カラム内径
7.7 mm
マトリックス
highly cross-linked agarose
平均部品サイズ
75 μm (d50v)
cleaning
3-14
working range
3-13
キャパシティ
27 mg binding capacity(27 mg BSA /ml (at QB10%))
適合性
suitable for bioprocess medium
詳細
Capto™ Phenyl (High sub) and Capto™ Butyl are hydrophobic interaction chromatography (HIC) media used in the capture and intermediate stages of protein purification.
アプリケーション
HiScreen™ columns are part of the process development platform available from Cytiva. The columns are prepacked with a range of BioProcess chromatography media (resins) and designed for method optimization and parameter screening. HiScreen™ columns have small bed volumes (4.7 ml), reducing the cost of sample and buffer consumption. The media used in HiScreen™ columns are also available in other prepacked formats and as bulk packs, for all scales of work from development and pilot studies to routine production.
Capto™ Butyl is intended for initial and intermediate step purifications requiring a chromatography medium with Low to medium hydrophobicity. Capto™ Butyl often works efficiently with rather Low salt concentrations. The mechanism of binding and elution onto the butyl ligand is different from that onto the phenyl ligand, giving a different selectivity. Performance during sample run and selectivity are similar to that of Butyl Sepharose™ 4 Fast Flow. Higher flow rates can be used with Capto™ Butyl during equilibration, wash and regeneration.
Capto™ Butyl is intended for initial and intermediate step purifications requiring a chromatography medium with Low to medium hydrophobicity. Capto™ Butyl often works efficiently with rather Low salt concentrations. The mechanism of binding and elution onto the butyl ligand is different from that onto the phenyl ligand, giving a different selectivity. Performance during sample run and selectivity are similar to that of Butyl Sepharose™ 4 Fast Flow. Higher flow rates can be used with Capto™ Butyl during equilibration, wash and regeneration.
特徴および利点
- Prepacked with Capto™ Butyl
- Excellent for method optimization and parameter screening due to the 10 cm bed height
- Easily connected in series to achieve 20 cm bed height
- Small bed volume for fast results and minimal sample/buffer consumption
- Reproducible results, scalable to BioProcess columns packed with the same media using the same flow velocity
保管および安定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
アナリシスノート
この製品の分析証明書は、www.cytiva.com.
法的情報
Capto is a trademark of Cytiva
HiScreen is a trademark of Cytiva
Sepharose is a trademark of Cytiva
シグナルワード
Warning
危険有害性情報
保管分類コード
3 - Flammable liquids
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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