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Merck

GE28-9893-35

Sigma-Aldrich

HiLoad® 16/600 Superdex® 200 pg

Cytiva 28-9893-35, column L × I.D. 60 cm × 16 mm, 34 μm avg. part. size

別名:

16/600 Superdex 200, HiLoad Superdex 200

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About This Item

UNSPSCコード:
41115700
NACRES:
SB.52

物質

bed support (polyamide)
glass column
glass fiber (polypropylene reinforced)

分子量

10000-600000 Da (globular proteins)

包装

pkg of 1 ea

メーカー/製品名

Cytiva 28-9893-35

パラメーター

<1.0 mL/min flow rate
<5 mL sample volume
0.3 Mpa (42 psi)

ベッド容積

120 mL

カラムL × 内径

60 cm × 16 mm

Matrix

agarose dextran composite
dextran-agarose particle platform
polymeric

マトリックス活性基

dextran-agarose phase

平均部品サイズ

34 μm

cleaning

1-14

動作範囲

3-12

分離法

size exclusion (SEC)

関連するカテゴリー

詳細

HiLoad® 16/600 Superdex® 200 prep grade are prepacked XK columns designed for high-resolution preparative gel filtration chromatography. The column is an excellent choice for separating MAbs from dimers and contaminants.

Superdex® prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component. Steep selectivity curves give excellent resolving power for peptides and proteins in the molecular weight ranges, Mr < 10 000 (Superdex® 30 pg), Mr 3 000 to 70 000 (Superdex® 75 pg), and Mr 10 000 600 000 (Superdex® 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions.

特徴および利点

  • High resolution with short run times and good recovery.
  • High capacity at high flow rates.
  • Separations can be scaled up to production levels.
  • Prepacked for convenience and reproducibility.
  • 1/16” fittings for convenient connections to AKTA design systems.

適合性

All commonly used buffers, 1 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile
for high-resolution preparative gel filtration chromatography, e.g, separate mAbs from dimers and contaminants

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

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法的情報

HiLoad is a registered trademark of Cytiva
Superdex is a registered trademark of Cytiva

ピクトグラム

Flame

シグナルワード

Warning

危険有害性情報

保管分類コード

3 - Flammable liquids


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資料

This page provides information about performing an isolation of recombinant protein complexes with different pull-down assays with products from GE Healthcare.

This page provides information about performing an isolation of recombinant protein complexes with different pull-down assays with products from GE Healthcare.

Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.

This page shows how to optimize purification of histidine-tagged proteins using GE Healthcare products.

プロトコル

This page shows how to perform a two-step purification of mouse monoclonal IgG1 using HiTrap Protein G HP from GE Healthcare for the capture step.

This page shows how to perform a two-step purification of mouse monoclonal IgG1 using HiTrap Protein G HP from GE Healthcare for the capture step.

AC separates proteins on the basis of a reversible interaction between a protein (or group of proteins) and a specific ligand coupled to a chromatography matrix. With such high selectivity and hence high resolution for the protein(s) of interest, purification levels in the order of several thousand-fold with high recovery of active material are achievable.

AC separates proteins on the basis of a reversible interaction between a protein (or group of proteins) and a specific ligand coupled to a chromatography matrix. With such high selectivity and hence high resolution for the protein(s) of interest, purification levels in the order of several thousand-fold with high recovery of active material are achievable.

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