おすすめの製品
物質
bed support (polyamide)
glass column
glass fiber (polypropylene reinforced)
分子量
10000-600000 Da (globular proteins)
包装
pkg of 1 ea
メーカー/製品名
Cytiva 28-9893-35
パラメーター
<1.0 mL/min flow rate
<5 mL sample volume
0.3 Mpa (42 psi)
ベッド容積
120 mL
カラムL × 内径
60 cm × 16 mm
Matrix
agarose dextran composite
dextran-agarose particle platform
polymeric
マトリックス活性基
dextran-agarose phase
平均部品サイズ
34 μm
cleaning
1-14
動作範囲
3-12
分離法
size exclusion (SEC)
関連するカテゴリー
詳細
Superdex® prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component. Steep selectivity curves give excellent resolving power for peptides and proteins in the molecular weight ranges, Mr < 10 000 (Superdex® 30 pg), Mr 3 000 to 70 000 (Superdex® 75 pg), and Mr 10 000 600 000 (Superdex® 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions.
特徴および利点
- High resolution with short run times and good recovery.
- High capacity at high flow rates.
- Separations can be scaled up to production levels.
- Prepacked for convenience and reproducibility.
- 1/16” fittings for convenient connections to AKTA design systems.
適合性
保管および安定性
アナリシスノート
法的情報
シグナルワード
Warning
危険有害性情報
保管分類コード
3 - Flammable liquids
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資料
This page provides information about performing an isolation of recombinant protein complexes with different pull-down assays with products from GE Healthcare.
This page provides information about performing an isolation of recombinant protein complexes with different pull-down assays with products from GE Healthcare.
Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.
This page shows how to optimize purification of histidine-tagged proteins using GE Healthcare products.
プロトコル
This page shows how to perform a two-step purification of mouse monoclonal IgG1 using HiTrap Protein G HP from GE Healthcare for the capture step.
This page shows how to perform a two-step purification of mouse monoclonal IgG1 using HiTrap Protein G HP from GE Healthcare for the capture step.
AC separates proteins on the basis of a reversible interaction between a protein (or group of proteins) and a specific ligand coupled to a chromatography matrix. With such high selectivity and hence high resolution for the protein(s) of interest, purification levels in the order of several thousand-fold with high recovery of active material are achievable.
AC separates proteins on the basis of a reversible interaction between a protein (or group of proteins) and a specific ligand coupled to a chromatography matrix. With such high selectivity and hence high resolution for the protein(s) of interest, purification levels in the order of several thousand-fold with high recovery of active material are achievable.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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