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GE28-9782-44

HiScreen Ni Fast Flow

Cytiva 28-9782-44

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About This Item

UNSPSCコード:
13111000
NACRES:
NA.56

包装

pkg of 1 ea

メーカー/製品名

Cytiva 28-9782-44

パラメーター

1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi

ベッドサイズ

7.7 mm × 100 mm

ベッド容積

4.7 mL

カラム内径

7.7 mm

Matrix

6% cross-linked agarose

粒径

45-165 μm

平均直径

90 μm

cleaning

2-14(Ni2+-stripped medium.)

動作範囲

3-12(Ni2+-stripped medium.)

キャパシティ

~40 mg binding capacity(histidine-tagged protein)

適合性

suitable for bioprocess medium

関連するカテゴリー

詳細

HiScreen columns prepacked with Ni Sepharose 6 Fast FLow for method optimization and parameter screening for purification of histidine-tagged proteins with Immobilized Metal ion Affinity Chromatography (IMAC).

アプリケーション

HiScreen columns are part of the process development platform available from Cytiva. The columns are prepacked with a range of BioProcess chromatography media (resin) and designed for method optimization and parameter screening. HiScreen columns have small bed volumes (4.7 ml), reducing the cost of sample and buffer consumption. The chromatography media used in HiScreen columns are also available in other prepacked formats and as bulk packs, for all scales of work from development and pilot studies to routine production.

HiScreen Ni FF is prepacked with the BioProcess chromatography medium Ni Sepharose 6 Fast FLow which has high binding capacities for
histidine-tagged Proteins and minimal Ni2+ leakage.

特徴および利点

  • High binding capacity, approx. 40 mg/mL medium and negligible leakage of Ni2+.
  • Prepacked, ready-to-use columns for convenient process development.
  • Excellent choice for method optimization and parameter screening due to the 10 cm bed height.
  • Easy connection in series to achieve 20 cm bed height.
  • Small bed volume gives fast results and minimal sample/buffer consumption
  • Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

法的情報

HiScreen is a trademark of Cytiva
Sepharose is a trademark of Cytiva

ピクトグラム

Environment

シグナルワード

Warning

危険有害性情報

保管分類コード

3 - Flammable liquids


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資料

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from GE Healthcare.

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using GE Healthcare products.

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using GE Healthcare products.

プロトコル

This page shows how to purify histidine-tagged recombinant proteins using Ni Sepharose 6 Fast Flow from GE Healthcare.

This page shows how to purify histidine-tagged recombinant proteins using Ni Sepharose 6 Fast Flow from GE Healthcare.

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

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