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使用法
sufficient for 250 purifications
包装
pkg of 250 ea
メーカー／製品名
Cytiva 28-9042-76
キャパシティ
>35 μg binding capacity
関連するカテゴリー
詳細
Tissue and cells genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from animal tissues and mammalian cell cultures, in just 90 min for tissues and 45 min for cells, using DNA spin columns.
Human and animal genomic DNA is found in the chromosomes and it is much longer and higher in molecular weight than plasmid DNA. Genomic DNA is the code for the genome, and this is what is needed to do genome sequencing.
For sequencing it is desirable that the genomic DNA extraction technique used allows the collection of intact genomic DNA in the longest fragments possible. A kit format can help standardize the procedures used to prepare tissue and cell genomic DNA samples for analysis.
To minimize the incidence of DNA shearing during extraction, Tissue and cells genomicPrep Mini Spin Kit includes a lysis solution with proteinase K to gently release genomic DNA from tissues or cells, including human genomic DNA.
The kit can be used for DNA isolation from diverse tissue types such as liver, kidney, and mouse tails with yields ranging from 0.5 to 1.5 μg of genomic DNA per mg of tissue. The kit consistently generates purified genomic DNA with optical density A260/A280 readings of 1.8 or higher.
The gDNA produced can then be further analyzed in PCR, for SNP genotyping, and next generation sequencing (NGS) projects.
Human and animal genomic DNA is found in the chromosomes and it is much longer and higher in molecular weight than plasmid DNA. Genomic DNA is the code for the genome, and this is what is needed to do genome sequencing.
For sequencing it is desirable that the genomic DNA extraction technique used allows the collection of intact genomic DNA in the longest fragments possible. A kit format can help standardize the procedures used to prepare tissue and cell genomic DNA samples for analysis.
To minimize the incidence of DNA shearing during extraction, Tissue and cells genomicPrep Mini Spin Kit includes a lysis solution with proteinase K to gently release genomic DNA from tissues or cells, including human genomic DNA.
The kit can be used for DNA isolation from diverse tissue types such as liver, kidney, and mouse tails with yields ranging from 0.5 to 1.5 μg of genomic DNA per mg of tissue. The kit consistently generates purified genomic DNA with optical density A260/A280 readings of 1.8 or higher.
The gDNA produced can then be further analyzed in PCR, for SNP genotyping, and next generation sequencing (NGS) projects.
特徴および利点
- Rapid procedure produces high molecular weight gDNA with minimal shearing.
- Capable of purifying up to 1.5 µg/mg of tissue from an input tissue sample range of 5 to 5NA mg, and up to 4NA µg of genomic DNA per 5 x 1NA6 cells depending on cell type.
- Total DNA extraction time reduced significantly to 9NA min.
- Produces high-quality genomic DNA with high molecular weight and less degradation.
- Verified in common downstream applications.
保管および安定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
アナリシスノート
この製品の分析証明書は、www.cytiva.com.
法的情報
illustra is a trademark of Cytiva
シグナルワード
Danger
危険有害性情報
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 3
最新バージョンのいずれかを選択してください：
資料
Polymerase chain reaction (PCR) is a technique that results in exponential amplification of a target DNA sequence.
プロトコル
This page shows genomic DNA purification from sample applied to FTA cards from GE Healthcare.
This page shows Genomic DNA purification using illustra™ genomicPrep Midi Flow Kits from GE Healthcare.
This page shows Genomic DNA purification using illustra™ genomicPrep Mini Spin Kits from GE Healthcare.
Nucleic Acid Sample Preparation Methodology
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